Lipid droplet: A functionally active organelle in monocyte to macrophage differentiation and its inflammatory propertiesAKTInflammationLipid dropletsRab5adifferentiationmacrophagemonocyteLipid droplets (LDs) perform several important functions like inflammatory responses, membrane trafficking, acts as secondary ...
macrophagelipid droplet accumulationACATDGATFrom a study on the biological activity of fungal stemphones and their derivatives, five derivatives having an O-alkyl moiety at C-11 of stemphone C were found to inhibit lipid droplet accumulation in macrophages without any cytotoxic effect. Among the ...
Fig. 3. Cellular localization of 15-LO-1 in 15-LO-1 RAW macrophage foam cells. Cells were incubated with acLDL to induce neutral lipid droplet formation. Lipid bodies were visualized by phase microscopy (A). 15-LO-1 was localized on lipid droplets using anti-15-LO-1 and an FITC-conjuga...
Sespendole was isolated as an inhibitor of lipid droplet formation in macrophages from the culture broth of a fungal strain Pseudobotrytis terrestris FKA-25. The compound inhibited the synthesis of cholesteryl ester and triacylglycerol by mouse macrophages with IC50 values of 4.0 and 3.2 M, respect...
The lipid droplet formation phenotype observed in macrophages was additionally confirmed in MEFs, where lipidomic analysis identified cholesterol esters as specifically downregulated in LAMTOR2 knockout cells. The data obtained indicate a function of LAMTOR2 in lipid metabolism....
macrophage states. Here, we present an inclusive reference atlas of human intraplaque immune cell communities. Combining single-cell RNA sequencing (scRNA-seq) of human surgical carotid endarterectomies in a discovery cohort with bulk RNA-seq and immunohistochemistry in a validation cohort (the ...
In addition, the potential of LDAPs as therapeutic targets to prevent the progression and/or facilitate the regression of the disease has been discussed. 展开 关键词: atherosclerosis cholesterol foam cell lipid droplet lipid droplet-associated proteins macrophage ...
increased with obesity. But intriguingly, genetic or pharmacological inhibition of lipophagy did not change lipid balance, indicating existence of another pathway critical to lysosome TG hydrolysis [138]. All these data indicate that the role of lipophagy in macrophage-driven inflammation may depend on...
Lipid droplet (LD) is a metabolically active organelle, which changes dynamically with the metabolic state and energy requirements of cells. Proteins that either insert into the LD phospholipid monolayer or are present in the cytoplasm, playing a crucial
Macrophage-derived foam cells play a pivotal role in atherosclerotic plaque formation by expressing scavenger receptors that regulate lipid uptake. However, the role of hypoxia in scavenger receptor regulation remains incompletely understood. Methods and results Using RT-qPCR, flow cytometry and immuno...