Class 11 Chemistry Concepts of Chemistry. Limiting Reagent. Limiting Reagent. In a chemical reaction, reactant whichis present in the lesser amount gets consumed after sometimeand after that no further reaction takes place whatever be the amount of the other reactant present. ...
In conclusion, we have presented a silver-mediated late-stage C-H trifluoromethoxylation reaction with arenes and heteroarenes as limiting reagent, which allows efficient late-stage trifluoromethoxylation of a variety of organic molecules and known drugs. Although the trifluoromethoxylation method reported...
For example, Ap4 deficiency resulted in a decrease in expression of the transcription factor Spdef. Interestingly, it was previously shown that ectopic expression of Spdef in the murine intestine promotes the differentiation of goblet cells, whereas it decreased the number of Paneth cells28. ...
For example, 10 μM rottlerin alone promotes apoptosis, but 5 μM rottlerin ameliorates etoposide-induced apoptosis in C6 glioma cells. Furthermore, rottlerin was reported to inhibit angiogenesis in a dose-dependent manner [47]. The application of rottlerin as potential pharmaceutical for ...
Slides were allowed to air dry for 30 min before applying hybridizing solution (70% formamide, 1 mg/ml blocking reagent (Roche), 10 mM Tris–HCl pH 7.2) containing diluted 1:250 FITC-OO-(CCCTAA)3 PNA probe (Panagene Cat. No. F1009). Spreads were denatured for 3 min at 80 °C on...
cat. no. C10619) following guidelines for cells with the following differences: permeabilization of tissues was performed overnight in PBS +0.3% Triton X-100, TdT reaction was performed by incubating overnight followed by 1.5 h at 37 °C, and incubation with Click-iT reagent was performed fo...
Total RNA was isolated from cells using TRizol reagent (Invitrogen) following the manufacturer’s protocol. Reverse transcription of 0.5–1 μg of total RNA was performed using random hexamers as primers and SuperScript III reverse transcriptase (Invitrogen) using the manufacturer’s protocol. Tra...
While aspects of the present disclosure can be described and claimed in a particular statutory class, such as the system statutory class, this is for convenience only and one of skill in the art will understand that each aspect of the present disclosure can be described and claimed in any sta...
for 2 h. The reducing sugars were measured using the dinitrosalicylic acid reagent solution (DNS) method, using a 2:1 DNS:starch assay ratio and boiled at 100° C. for 5 mins. The absorbance is measured at 540 nm. Intracellular trehalose assay. For evaluation of intracellular trehalose ...
for 2 h. The reducing sugars were measured using the dinitrosalicylic acid reagent solution (DNS) method, using a 2:1 DNS:starch assay ratio and boiled at 100° C. for 5 mins. The absorbance is measured at 540 nm. Intracellular trehalose assay. For evaluation of intracellular trehalose ...