We acutely depleted PtdIns3P by inhibiting the PtdIns3P-kinase class III (VPS34) with the highly specific inhibitor SAR40538. As expected, HRS recruitment to EGF-positive endosomes was strongly reduced, as was the downstream ESCRT component CHMP4B (Fig. 6a–c). ESCRT waves were unchanged in...
Therefore, we next sought to identify subpopulations of T cells associated with response, by performing mass cytometry (CyTOF) on baseline (PRE) melanoma tissue dissociates from patients treated with anti-PD-1 monotherapy (n = 11) or combined immunotherapy (n = 7), using a customized panel ...
Both the N- and C-terminal membrane helices in Orai1 are shown to be regions of interaction with STIM1 (refs 11,22), and both are clearly necessary for STIM1-induced channel activation22–28. However, the molecular site within the STIM1 molecule that interacts with Orai channels and how...
al., eachEPAshows a specific binding pattern pointing to functional differences among the different genes [40]. When clusteringEPAsbased on their different binding affinities,EPAswere classified in three different groups.EPA9andEPA10belong to class I, and share this class withEPA1. When tested to...
REAGENT or RESOURCESOURCEIDENTIFIER Antibodies WIZ antibody Novus Biologicals Cat#NBP1-80586; RRID: AB_11011659 CTCF antibody Active Motif Cat#61311; RRID: AB_2614975 SMC1A antibody Bethyl Laboratories, Inc Cat#A300-055A; RRID: AB_2192467 RAD21 antibody Bethyl Laboratories, Inc Cat#A300-080A; ...