Library preparation and MiSeq sequencing for the genotyping-by-sequencing of the Huntington disease HTT exon one trinucleotide repeat and the quantification of somatic mosaicismHuntington disease \\(HD) is an autosomal dominant neurodegenerative disorder caused by the expansion of a CAG repeat in the ...
Sequencing platform and library preparation choices impact viral metagenomes. BMC Genomics 14: 320.Solonenko SA; Ignacio-Espinoza JC; Alberti A;.Sequencing platform and library preparation choices impact viral metagenomes.BMC Genomics.2013.320-
Provided in the present invention is a sequencing library and a preparation method thereof, wherein the inserted fragment of the sequencing library is a concatemer in which the sequences to be tested and tag sequences are alternated along the same direction. Also provided is a sequencing method...
Diagenode offers a comprehensive product portfolio for library preparation such as CATS RNA-seq Library Preparation Kits,ChIP-seq and DNA sequencing library preparation solutions
2. Sequencing scenarios 3. Automation processes in NGS library preparation 4. Automation solutions 5. Implementation and application aspects 6. Conclusion and outlook Declaration of Competing Interest Acknowledgements ReferencesShow full outline Cited by (119) Figures (5) Tables (1) Table 1Biotechnology...
D-Plex technology is the latest innovation in RNA-sequencing. This ultrasensitive, ligation-free method utilizes the capture and amplification by tailing and switching for library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for ...
Library preparation is the first step of next generation sequencing. It allows DNA or cDNA to adhere to the sequencing flow cell and allows the sample to be identified. Two common methods of library preparation are ligation-based library prep and tagmentation-based library prep. Once your librari...
DNA samples were previously extracted from whole blood for Samples 1–4 and from isolated white blood cell subsets for Samples 5–8 (CD4+ T cells: Samples 5 and 8; Neutrophils: Samples 6 and 7). Library preparation and sequencing protocols were carried out by two independent sequencing ...
Optimize your enterprise site with iGeneTech’s NGS library preparation kits. Our comprehensive solutions include DNA and RNA library construction, WGS library preparation, and detailed NGS library preparation protocols. Learn more about our NGS library
One-step RT-PCR protocol for amplicon-based viral genome sequencing Post-vaccination immune repertoire profiling using the SuperScript IV Template-Switching RT Master Mix for 5´ RACE How to sequence single- and dual-indexed libraries together Library preparation from circulatin...