Next-generation sequencingDNA bindingChromatin immunoprecipitation with massively parallel DNA sequencing (ChIP-Seq) has been used extensively to determine the genome-wide location of DNA-binding factors, such as transcription factors, pdoi:10.1007/978-1-4939-3378-5_9Karyn L. Sheaffer...
Diagenode offers a comprehensive product portfolio for library preparation such as CATS RNA-seq Library Preparation Kits,ChIP-seq and DNA sequencing library preparation solutions
As previous studies have focused on comparing established pre- and post-bisulfite WGBS library methods as well as short-read sequencing using older HiSeq platforms and high PhiX spike-in10,11,12, there remains a wide knowledge gap for comparison of library preparation method performances with respe...
Whether you are studying the human genome, investigating the microbiome, or exploring the diversity of life on earth, Oxford Nanopore library preparation solutions offer a flexible, cost-effective, and streamlined way to generate high-quality sequencing
Considerable emphasis will need to be placed on standardisation to ensure robustness and reproducibility. This review presents an overview of the current state of automation of library preparation for next generation sequencing. Major challenges associated with library preparation are outlined and different ...
Library preparation is the first step of next generation sequencing. It allows DNA or cDNA to adhere to the sequencing flow cell and allows the sample to be identified. Two common methods of library preparation are ligation-based library prep and tagmentation-based library prep. Once your librari...
Most current NGS library preparation protocols introduce significant biases.Simple modifications of existing protocols are often sufficient to reduce bias.PCR is a major source of bias but Kapa HiFi polymerase performs remarkably well.RNA-seq is particularly bias-prone; the best protocol may be the dU...
RNA-Sequencing (RNA-Seq) of peripheral blood can be a valuable source of information for investigating the status and mechanism of diseases. However, blood contains 50–80% unwanted hemoglobin (Hb) transcripts. Lexogen’s QuantSeq mRNA-Seq-Kit for Illumina RNA-Seq features a ‘Globin Block’ ...
Following library preparation and sequencing on a NextSeq 500 instrument, relative abundances were estimated by pseudo-alignment of reads against the reference genome sequences using kallisto [27], based on its near-perfect accuracy using simulated sequencing reads (see Methods). For all protocols, ...
Current protocols for preparing DNA for next-generation targeted sequencing are labor-intensive, require large amounts of starting material, and are prone to artifacts that result from necessary PCR amplification of sequencing libraries. Typically, sample preparation for targeted NGS is a two-step ...