Bioinformatics cDNA library construction and sequencing of the soft coral Eunicea fusca UNIVERSITY OF HOUSTON-CLEAR LAKE Lory Santiago-Vazquez CarreonDesirelysCoral reefs have enormous economic and environmental value. However, their habitat is being threatened by stressors such as abnormal ocean ...
sequencing refers to the library construction of qualified DNA/RNA samples provided by partners by using scientific standard experimental methods, or the qualified library provided by customers by using PacBio Sequel/Sequel II platform to obtain high-quality sequencing data for subsequent bioinformatics ...
For transient expression inNicotianaspp., the coding sequences of AvrSr13 and AvrSr22 were transferred from the library constructs pTA22-0336-PBS and pTA22-0100-PBS, respectively, into the pDONR207 vector via Gateway BP reaction (Invitrogen). The predicted coding sequences ofSr13c,Sr22andAvr...
This unit presents protocols for construction of next-generation sequencing (NGS) directional RNA sequencing libraries for the Illumina HiSeq and MiSeq from a wide variety of input RNA sources. The protocols are based on the New England Biolabs (NEB) small RNA library preparation set for Illumina...
SEQUENCING LIBRARY CONSTRUCTION KIT AND USE METHOD 专利名称：SEQUENCING LIBRARY CONSTRUCTION KIT AND USE METHOD AND APPLICATION THEREOF 发明人：LUO, Junfeng,罗俊峰,WANG, Jinping,汪进 平,GUO, Changquan,郭长全 申请号：CN2019/127004 申请日：20191220 公开号：WO2020/135259A1 公开日：20200702 专利内容由...
circulating DNAlow inputsequencing library preparationCirculating DNA is cell-free DNA (cfDNA) in serum or plasma that can be used for non-invasive prenatal testing, as well as cancer diagnosis, prognosis, and stratification. High-throughput sequence analysis of the cfDNA with next-generation ...
Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. Results Here, we report the construction and characterization of the CHORI-231 BAC library constructed from a Danish-farmed, male American mink (Neovison vison...
In this chapter we describe the construction of cDNA libraries for (1) RNA deep sequencing (RNA-seq) and (2) TSS mapping using the Illumina technology. 展开 关键词: Legionella pneumophila Next-generation sequencing Transcriptional start site mapping Small ncRNA ...
A low-redundancy cDNA library was generated for the tropical scallop Nodipecten nodosus.About 250 unique EST were catalogued and sequences were functionally annotated.Genes related to apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity were revealed.关键词:...
Exploring the unmapped DNA and RNA reads in a songbird genome From trash to treasure: detecting unexpected contamination in unmapped NGS data 转录组测序(RNA-seq)详细建库步骤与原理- 里面的几个图不错 RNA sequencing: the teenage years A survey of best practices for RNA-seq data analysis ...