First, pT7CACT1-derived plasmid expressing HlyC was constructed by replacement of the cyaC gene from its start to stop codon in pT7CACT1 by hlyC. Second, an overlapping PCR mutagenesis procedure was used to amplify DNA segments encoding required CyaA/HlyA hybrid molecules and the PCR ...
toxins Article Aggregatibacter actinomycetemcomitans Leukotoxin Is Delivered to Host Cells in an LFA-1-Indepdendent Manner When Associated with Outer Membrane Vesicles Justin B. Nice 1, Nataliya V. Balashova 2 , Scott C. Kachlany 3, Evan Koufos 1, Eric Krueger 1, Edward T. Lally 2 and ...