For production and subsequent purification of HlyC-acylated HlyA, the cyaA gene in the pT7CACT1-derived plasmid harboring hlyC was replaced from its start to stop codon by the entire hlyA gene, which was fused in-frame at the 3′ terminus to a sequence encoding a double hexahistidine ...
toxins Article Aggregatibacter actinomycetemcomitans Leukotoxin Is Delivered to Host Cells in an LFA-1-Indepdendent Manner When Associated with Outer Membrane Vesicles Justin B. Nice 1, Nataliya V. Balashova 2 , Scott C. Kachlany 3, Evan Koufos 1, Eric Krueger 1, Edward T. Lally 2 and ...