kit, lentiviral vector particles, plasmid vector composition, plasmid vector, chimeric hiv-1 derived antigen, vsv-g envelope protein, nucleic acid molecules, immunogenic composition and use of a lentiviral vector the present invention refers to relates to the design of gene transfer vectors and ...
Since we demonstrated that the transgene expression was mainly localized at the site of i.m. injection, we then focused on the T-cell activating properties of APCs, notably the dendritic cell subsets in the pLNs of lentiviral vector-immunized mice. To identify the immune cell subsets transduced...
Full-length dystrophin delivery with template-switching vector. (a) Schematic for the modified dystrophin vector. DNA synthesis must initiate on the 5′ strand and strand transfer to the 3′ LTR will then permit synthesis of a functional provirus following template-switching within the region of d...
The pSIV-T1+ plasmid29 encodes a packaging-competent SIVmac251-based vector that expresses the enhanced green fluorescent protein (GFP) marker gene under control of the CMV promoter (Figure 1). Fig. 1. Generation of SIVmac251-derived vectors. / The genome of an infectious molecular clone of...
Lentivirus vectors were produced by transient cotransfection of 293T cells with the backbone vector plasmid derived from the pHR series, the packaging plasmid pCMV-ΔR8.9, and the plasmid pMD.G encoding the VSV-G envelope, as described previously.10, 19 Viral titer was determined by assessing ...
Lentiviral vectors pseudotyped with vesicular stomatitis virus-glycoprotein (VSV-g) were generated from the transient co-transfection of the newly-engineered SIN transfer vector, second-generation LV packaging construct pCMVΔ8.91 [30], envelope plasmid pMD.G [31], and pAdVAntage™ vector [32] (...
FIG. 4: Analysis of Vpr-Cre fusion protein activity. The stable cell line HT1080/Gβ, which had previously been transduced with a retroviral vector containing both GFP and β-gal genes, was co-transfected with either a Cre or a Vpr-Cre expression plasmid. A. Basic schematic of the retrov...
If a lentiviral transfer plasmid contains a single restriction site, one can use standard cloning techniques to ligate the insert into this site. If it is not immediately feasible to digest and clone the insert from a parent vector, some possible approaches to using this site include subcloning...
9. A lentiviral vector, which comprises plasmid pTRIP/sEWNV. 10. A host cell transfected or transduced with the lentiviral vector of claim 9. 11. A method of immunizing against a pathogenic agent comprising administering a composition as claimed in claim 1 to an animal in need thereof, wher...
High titers of lentiviral vectors are required for the efficient transduction of a gene of interest. During preparation of lentiviral the vectors, the protein of interest is inevitably expressed in the viral vector-producing cells. This expression may af