GENETIC transductionELECTROPORATIONCATIONIC polymersGENE expressionNUCLEAR membranesBackground: Transfection is an important analytical method for studying gene expression in the cellular environment. There are some barriers to efficient DNA transfection in host cells, including circumventing the plasma membrane,...
Using a lentiviral vector, we will perform: (1) in vitro transduction of cultured neonatal rat myocytes; (2) in vitro transduction of cultured adult rabbit myocytes; (3) in vivo transduction by direct epicardial injection ... JD Bakker,H Tan 被引量: 0发表: 2005年 A high-titer lentiviral...
we first developed a lentiviral vector harboring the human β2-microglobulin (β2m) promoter. We showed that intramuscular (i.m.) immunization with lentiviral vector employing the β2m promoter led to highly efficient in vivo dendritic cell transduction and in...
Viral vector production is performed by transient transfection, packaging cell lines or transduction. Human embryonic kidney 293 cells are widely used for viral vector production because of high transfectability and adaptability.9 The human embryonic kidney 293 variant 293T10,11 is especially efficient ...
The authors used quantitative PCR to compare the efficiency of plasmid transfection or viral transduction, and found that the rate of Flp-mediated integration from 2-LTR circle was around 60 times more efficient compared to plasmid. Possible reasons for the greater efficiency of 2-LTR-circle-...
Lentiviral vectors were produced by transient transfection of 293T cells as described previously [45], [46]. Vector-containing supernatants were harvested 48 h after transfection, filtered through a 0.45-μm syringe filter, and then stored at − 80°C. Infectious titers were determined using 293...
Moreover, lentiviral delivery does not produce the non-specific cell responses typically associated with chemical transfections or use of an adenoviral delivery system (Gould, 2003, Cann, 2000). SBI offers GeneNet™ siRNA libraries constructed in both FIV-based and HIV-based lentivectors. SBI's...
Production of LVs and transduction of cell lines Lentiviral vectors pseudotyped with vesicular stomatitis virus-glycoprotein (VSV-g) were generated from the transient co-transfection of the newly-engineered SIN transfer vector, second-generation LV packaging construct pCMVΔ8.91 [30], envelope plasmid ...
High-titer lentiviral vectors are required for efficient transduction and time- and cost-savings. Lentiviral vectors are usually produced in 293T cells with the transfection of self-inactivating expression and helper plasmids [2]. Because most lentiviral vectors contain ubiquitously active promoters, the...
Generation of VSV-G pseudotyped lentiviruses 2.5 × 105cells/ml of 293T cells were plated in 2 ml/well in a 6-well plate 16 hours prior to transfection. The VSV-G pseudotyped HIV-1 WT-GFP, HIV-1 G89V-GFP mutant, HIV-1 WT-Luc, SIVmac-Luc, and SIVagmTAN-Luc were generated as...