(1998). Interruption of T-cell signal transduction by lentivirus lytic peptides from HIV-1 transmembrane protein. J. Pept. Res. 51(1), 75-59.Beary TP,Tencza SB,Mietzner TA,Montelaro RC.Interruption of T-cell signal transduction by lentivirus lytic peptides from HIV-1 transmembrane protein....
Tumour immune evasion presents a significant challenge to the effectiveness of cancer immunotherapies. Recent advances in high-throughput screening techniques have uncovered that loss of antigen presentation and cytokine signalling pathways are central m
基因转导(Gene Transduction)的稳定性是实现效应细胞稳定表达CAR的前提。当前常用的CAR-NK转导技术包括病毒载体和非病毒载体两类,其中常用的病毒载体包括逆转录病毒(Retrovirus)、慢病毒(Lentivirus)和腺相关病毒(Adeno-Associated Virus, AA...
For human primary T cell gene knockdown, we adopted lentiviral delivery of short hairpin (sh)RNAs with lentiviral gene product X (Vpx) containing virus-like particles (VLPs) to efficiently transduce lentivirus into unstimulated primary human naive T cells28,29 (Fig. 3b). Spinoculation with Vpx-...
Following 1 day of rest after blasticidin selection, KRAB-dCas9 expressing cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, 11131D), at 1:1 cell:bead ratio. Sixteen to 20 h after activation, cells were transduced with a CRISPRi CROPseq lentivirus encodi...
In one embodiment, the T cell has been transduced with a lentivirus vector that expresses a human surface reporter such as human SSTR2, PSMA, or NIS. The present invention provides a lentivirus vector comprising an elongation factor 1a promoter and a nucleic acid molecule encoding SSTR2, PSMA,...
Tisagenlecleucel (CTL019), an anti-CD19 CAR-T cell product of single infusion, enables the lentiviral transduction of each patient autologous T cells with a gene encoding a second-generation anti-CD19 CAR harboring a 4-1BB co-stimulatory domain [24]. The approval was clinically supported ...
碧云天同时提供基于CRISPR/Cas9技术的ZAP70基因敲除的质粒(L16800 pLenti-ZAP70-sgRNA)、慢病毒(L16801 ZAP70 Knockout Lentivirus)、HEK293T细胞(L16802 ZAP70 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L16803 ZAP70 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L16804 ZAP70...
碧云天同时提供基于CRISPR/Cas9技术的GPR52基因敲除的质粒(L27340 pLenti-GPR52-sgRNA)、慢病毒(L27341 GPR52 Knockout Lentivirus)、HEK293T细胞(L27342 GPR52 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L27343 GPR52 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L27344 GPR52...
We then analyzed CD276 surface expression on BC cell lines of four different molecular subtypes: luminal A-like, luminal B-like, HER2+, and triple-negative. High surface expression levels were observed with all four cell lines (Fig. 2A). The number of CD276 molecules per cell was between ...