Slides were observed either under an Eclipse Nikon microscope or Leica laser confocal microscope (Leica Microsystems, Germany). ScIentIfIc REPOrTs | 7: 13580 | DOI:10.1038/s41598-017-13202-9 9 www.nature.com/scientificreports/ In vivo tracking experiments with Acetylated Low Density Lipoprotein, ...
Biotin-labeled embryos were permeabilized, blocked with 10% normal goat serum, and stained with an avidin/horseradish peroxidase complex (ABC Kit; Vector) using diaminobenzidine as substrate. Labeled embryos were examined on a Nikon E800 fluorescence microscope, and some optically sectioned with a ...