Cell division is marked by a series of distinct cellular events. To study cell cycle, we cultured adult Rosa-NICD cochleae and infected them with ad-Myc/ad-Cre(Fig.2a). We identified SCs in mitosis by mitotic figures and MKI67 labeling (Fig.2b–d, h). Dividing SCs were labeled with ...
The cochlea is innervated by type I and type II afferent neurons. Type I afferents are myelinated, larger diameter neurons that send a single dendrite to contact a single inner hair cell, whereas unmyelinated type II afferents are fewer in number and rec
the proliferative cells were observed under scanning electron microscope and transmission electron microscope.Results ①The cell spheres can be observed in the cell culture of the Corti's organ.In present experiment,90.1% of cells in spheres were labeled by BrdU,while nestin of spheres,the marker ...
In addition, crossover controls on the confocal microscope were performed to ensure that the fluorescent probe signal from one channel was not bleeding through to the other. In this case, tissues labeled only with fluorescein were scanned in the rhodamine channel and tissues labeled only with ...
Under a Creative Commons license open accessHighlights Summary In mammals, hearing loss is irreversible due to the lack of regenerative potential of non-sensory cochlear cells. Neonatal cochlear cells, however, can grow into organoids that harbor sensory epithelial cells, including hair cells and supp...
(P)3 and P30 WT mice and immunolabeled them with the HC marker Myosin7a, the supporting cell (SC) marker Sox2, and DAPI to label the DNA. We first observed the cochlear basilar membrane under a 5 × microscope (Fig.1A). Immunofluorescence staining showed that YAP was expressed in ...
(Microm, Thermo Fisher Scientific, Walldorf, Germany) and were immediately mounted on SuperFrost® Plus microscope slides (Langenbrinck, Emmendingen, Germany). For lateral wall whole-mount preparations, the otic capsule was carefully removed, and the lateral wall was then separated from the OC in...
Decalcified cochlea was cryopreserved and 20-μm frozen mid-modiolar sections were immunolabeled for immune cells. The whole-mount preparations and sections were stained using the following antibodies at 1:50 to 1:100 dilution: goat anti-mouse CD45 (AF114, R&D Systems; a kind gift from Dr....
60 in blocking solution), and nuclei were stained with Hoechst 33342 (Molecular Probes, catalog #H3570, 10 mg/ml stock diluted 1:500 in blocking solution). Tissues were mounted in Fluoromount-G (Southern Biotech) and imaged on an LSM 780 laser scanning confocal microscope (Carl Zeiss ...
Hesr1 is expressed as a broad band of labeled cells in the ventral side of the developing cochlear duct (Figs. 1A, D), while Hesr2 is expressed as a narrower band of labeled cells, nested in the Hesr1 expressing region (Figs. 1B, E). At E14.5, in basal regions of the cochlea, ...