Code Issues main 1Branch 2Tags Code Repository files navigation README LeafNet Description LeafNet is a convenient tool that can robustly localize stomata and segment pavement cells for light-microscope images of leaves. Installation LeafNet has been tested under Linux, Mac OS Catalina (10.15), an...
The beating videos of the hiPSC-CM LVD constructs were recorded by the inverted microscope. HUVEC cells pre-labeled with a red cell tracker were encapsulated into fluorescent fibrinogen suspension to characterize the dynamic formation process of tubular structures in LVD tissues. The fluorescent ...
Optical photographs of the sections were captured with an Olympus BX63 microscope. To better analysis the expression level and pattern of CaLEAFY and CaARF23 between WT and mpl1-1, the paraffin sections of WT and mpl1-1 were placed on the same slide for hybridization. Bulk segregation ...
The water fern strainCeratopteris richardiiHnn, gifted by Andrew Plackett from Jane Langdale’s lab, was used. Spores were first imbibed under darkness for two days, and then sterilized using a 1∶5 diluted commercial bleach for 30 min under rocking, and finally washed five times using autoclave...
The coverslips were then washed and incubated with fluorescein-labeled secondary antibody for 1 h at room temperature in the dark. Finally, the cells were counterstained with DAPI and examined under a fluorescence microscope (OLYMPUS BX53, Japan). The image J software was used to measure the ...
Before RNA extraction, samples were carefully examined under a stereo microscope for the presence of ant eggs, larvae, and pupae, which were all quickly removed using a pair of forceps. Additionally, RNA was extracted from three samples of the PDA pure cultures and five samples of the WBA ...
For histopathology analysis, lung and liver tissues were fixed by paraformaldehyde and then were dehydrated, embedded in paraffin and cut into serial sections at 5 μm. Sections were stained with hematoxylin and eosin (H&E) solution and observed them under an optical microscope (DP73, OLUMPUS,...
Xanthomonas oryzae pv. oryzae (Xoo) injects major transcription activator-like effectors (TALEs) into plant cells to activate susceptibility (S) genes for promoting bacterial leaf blight in rice. Numerous resistance (R) genes have been used to construct
The image of cells invaded through the membrane was capture and counted under the light microscope. 2.10. Cell Growth Curve Analysis VSMC was seeded into a 6-well culture plate at a density of 7 × 104 cells/mL, and then incubated with TNF-α (10 ng/mL) in the absence or presence ...
The sellotape with its imprint was peeled from the leaflets and placed onto a glass microscope slide. Replicas were examined under an optical microscope (OPTICA AXIOM 7000). Pictures were digitally recorded for each slide at magnifications ×100 and ×400 and used for stomata measurements. The ...