for a specific leaf sample is dictated by the surface features that need to be preserved (such as trichomes, epidermal cells or wax microstructure), the resolution to be achieved, availability of the appropriate processing equipment and the technical capabilities of the available electron microscope....
After drying in darkness at laboratory temperature, leaf segments were placed on a glass slide, covered with a cover slip and observed under a microscope with UV illumination (Nikon, Eclipse 80i). The effect of different treatments on hyphal growth was assessed at 1 dpi by determining the ...
The inducibility of the pC-HSL receiver for plants grown in soil was tested (Fig.2d, e). Seeds were sterilized and germinated on agar plates to achieve uniform germination. After the emergence of the first leaf, the plants were transferred into 1:3 vermiculite:soil non-sterile mix supplemente...
For subcellular localization analyses, leaf segments of transformed tobacco plants were cut 72 h or 96 h after infiltration and observed under the confocal microscope. To analyze the binding of organelles to SpyCatcher-beads, the beads were diluted in appropriate buffer prior to microscopic analysis....
Thus, the viral particles observed in an electron microscope are immobile structures (virions). They are absent from the vegetative phase during intracellular reproduction of virions. Mature viral particles of the next generation produce, in the infected cells, viral inclusions in the form of variou...
The rhizosheath-root system is an adaptive trait of sandy-desert speargrasses in response to unfavourable moisture and nutritional conditions. Under the deserts’ polyextreme conditions, plants interact with edaphic microorganisms that positively affect
tests or nucleic acid probe tests against known viruses. In sap from infected cells and in purified preparations, most viruses and viroids can be seen under the electron microscope. Morphology Nearly half of the known viruses are rigid rodlike, 15×300nm (nanometers) (Figure 20(a)), or ...
and washed with water. Safranin O staining was performed by immersing the sections in Safranin O for 2 h. The sample was decolorized using gradient ethanol and fixed in plant solid green staining solution. The tissue sections were mounted with neutral balsam and observed under a microscope. Pano...
The third leaf middle area of 14-d-old plants were transversely sectioned and stained by IOSA method. Different cell types are indicated: msc, mestome sheath cell; cc, companion cells; mx, metaxylem; px, protoxylem; stec, sieve tube element cells; xpc, xylem parenchyma cells; vbsc, ...
(Extended Data Fig.2).P. herbarumis known as a plant pathogen causing leaf spot in various crops27. LikeD. spatulata,A. crateriformecan be cultivated under laboratory conditions using potato dextrose agar (PDA) and Murashige and Skoog (MS) agar. The acidophilicA. crateriformegrows optimally ...