Fig. 1- 4 Principle of triple quadrupole mass spectrometry. 11 华中农业大学2020 届硕士研究生学位 (毕业)论文 3.3 衍生化质谱技术 LC-MS 联用技术由于其固有的灵敏度和选择性,已成为最重要的分析技术之一。 但是,仍有许多难电离或难破碎的化合物不能通过LC-MS 很好地分析,因为这会使 检测灵敏度极低。而...
ESI is a complex process comprises of many different variables, no set of parameters will guarantee large signal intensity MS Structure elucidation Basic principle Nitrogen Rule Isotope ratio (Cl, Br, etc.) Adduct ions LC/MS Post column addition (acid or base) MS/MS or CID : from fragments ...
Principleinvestigator,PI主要研究者 Lastobservationcarryforward,LOCF最接近一次观察的结转LC-MS--第4页 Lastobservationcarryforward,LOCF最接近一次观察的结转LC-MS--第5页 Productlicense,PL产品许可证 Protocol试验方案 Protocol试验方案 Protocolamendment方案补正 Qualityassuranceunit,QAU质量保证部门 Qualityassurance,QA质...
In principle, there are two modes of ion exchange separation: cation and anion exchange. Combining SCX with RP chromatography offers high orthogonality, as SCX has a high loading capacity while RP can achieve high resolution separations amenable to LC–MS analysis and both involve different ...
Liquid chromatography mass spectrometry technique(LC-MS) was adopted to analyze the metabolites. A total of 6 982 metabolites were detected,including 2 704 negative ions and 4 278 positive ions. Principle component analysis (PCA) displayedthat Lycium ruthenicum Murr. and Lycium barbarum L. could ...
In principle the charge states during liquid chromatography influence the charge distribution of the analytes in the MS. The same holds in changing electrospray conditions such as voltage, application of shearing gas (ionspray), different eluent or uses of eluent modifiers etc). Charge state differen...
Lastly, although the database currently focuses on marine bacteria, it can in principle be extended to other organisms such as human pathogens, fungi and plants. The database manage- ment software is free and open-source and can be similarly deployed to develop LC-MS fingerprint databases for ...
曲线群基本围绕O位点延伸,提 示对每个患者而言,Gepts系统基本上能够准确靶控血浆舒芬太尼浓度. 3.3模型间的比较 Gepts参数和Slepchenko’S参数在预测血药浓度时均具准确性,而Slepchenko 系统的摆动度(wobble)较小. 结论 1.本实验建立了一种高敏UPLC-MS/MS方法来定量检测舒芬太尼血药浓度. 2.在多次变换目标...
The established LC-MS/MS workflow offers a comprehensive analysis of a wide range of organic acids, acylcarnitines and acylglycines in urine to perform effective, rapid and sensitive semi-automated diagnosis of more than 80 IMDs. Keywords: inherited metabolic disorders; liquid chromatography; mass sp...
The protocol enables molecular cartography to be performed in any mass spectrometry laboratory and, in principle, for any spatially mapped data. We anticipate applications, in particular, in medicine, ecology, agriculture, biotechnology, and forensics. The protocol takes 78 h for a molecular map of...