最后利用LC-MS/MS进行检测定量。 细心的读者可能已经发现,总抗体的测定与DAR值分析的第一步处理基本一致,实际上唯一的区别在于使用LC-MS/MS平台测定总抗时,由于对捕获试剂的特异性要求下降,可以选择的捕获试剂除了抗人抗体,特异性抗体,抗原蛋白之外,还可以使用通用性更强,价格相对较低,特异性较差的Protein A/G以...
近日,合肥工业大学/北方民族大学魏兆军教授团队在Food Chemistry (Q1,IF: 9.231)期刊上发表了题为“LC-MS based metabolomics analysis of okara fermented by Bacillus subtilis DC-15: Insights into nutritional and functional profile”...
通过使用网站数据库(人类代谢组数据库[HMDB],Metlin,MassBank,MassBank of North America[MONA]等)搜索,人们可以通过使用LC-MS/MS获得代谢标志物的MS/MS谱图,以进一步确认其化学特性。由于代谢物定量(单个样品的LC-MS扫描)和代谢物结构(混合样品的LC...
LC-MS-based metabonomics analysis (vol 866, pg 64, 2008) 中国科学院机构知识库(CAS IR GRID)以发展机构知识能力和知识管理能力为目标,快速实现对本机构知识资产的收集,长期保存,合理传播利用,积极建设对知识内容进行捕获,转化,传播,利用和审计的能力,逐步建设包括知识内容分析,关系分析和能力审计在内的知识服务...
An LC-MS/MS-based approach that employs authentic radioactive metabolites as reference standards was developed to estimate metabolite exposures in early drug development studies. This method is useful to estimate metabolite levels in studies done with non-radiolabeled compounds where metabolite standards ...
2020年11月华南农业大学张志祥课题组在Scientific Reports期刊上发表题为“Integrated LC–MS and GC–MS based untargeted metabolomics studies of the effect of Azadirachtin on Bactrocera dorsalis larvae”的研究成果。通过LC-MS和GC-MS的非靶向代谢组学研究方法,研究分析内源性代谢物的变化以及印楝素对背侧双歧杆...
基于LC/ MS代谢组学评价人参总皂苷对模拟微重力所导致记忆缺失的改善作用 期刊:Journal of Pharmaceutical and Biomedical Analysis,IF=3.24 发表时间:2016.4.1 01 研究背景 微重力(MG)会引起大脑的变化,这可能是人类和实验动物许多记忆障碍的根本原因。微重力(MG)可阻碍海马神经发生,改变脑基因和蛋白(如神经肽)的表...
Liquid chromatography (LC) coupled with mass spectrometry (MS) is widely used for the determination of mycotoxins in cereals and cereal-based products. In
Prioritization of putative metabolite identifications in LC‐MS/MS experiments using a computational pipeline One of the major bottle-necks in current LC-MS-based metabolomic investigations is metabolite identification. An often-used approach is to first look up me... B Zhou,JF Xiao,HW Ressom - ...
2015年,Richard Rogers等发表了第一篇基于LC-MS的Multi-Attribute Method(MAM)方法应用于单克隆抗体的论文,通过肽图分析手段,在氨基酸序列水平获得更详细的结构信息,…