Fig.1. Human iPS cells on laminin-511 E8 (coated laminin concentration 0.4 mkg(micrograms)/cm²): ZO-1, Beta-Actin and Objective lens; X40) Why the E8 Fragment? There are more than 12 Laminin isoforms, all of which promote stem cell growth. Laminin-511 is a major component of the ...
Laminin-511 E8,通常简称为LN511-E8,是一种特定形式的层粘连蛋白,为细胞提供结构支持并促使细胞彼此粘附。LN511-E8可以应用于诱导多功能干细胞(iPSC)的培养,它提供了一个支持性的基质,使得iPSCs能够粘附在培养皿表面上并进行增殖。这种基质能够通过与细胞表面受体的相互作用,促进细胞的黏附和扩增。Laminin511-E8无...
Laminin-511 E8,LN511-E8,511E8 蛋白序列: DNA序列编码人O15230-1 (Laminin-511 alpha5 E8) & P07942-1 (Laminin-511 beta1 E8) & P11047-1 (Laminin-511 gamma1 E8),带有His标签在C端 分子量: 预测分子量为76.7 kD,35.0 kD,35.7 kD
层粘连蛋白511 E8是一种截短的层粘连蛋白511,由α5、β1 和γ1 链的羧基端组成。Laminin-511 E8作为功能最小的形式,保留了与整合素结合的全部能力。LN511-E8为细胞提供结构支持并促使细胞彼此粘附。LN511-E8可以应用于诱导多功能干细胞(iPSC)的培养,它提供了一个支持性的基质,使得iPSCs能够粘附在培养皿表面上...
本制品是CHO-S细胞(Life Technologies)表达的重组人层粘连蛋白511-E8片段。 保存和稳定性 该产品可在2～15℃条件下稳定保存至标签上标示的有效期限内。避光保存。 iMatrix-511在4℃下稳定。 该产品的有效期为自制造日期开始2年。 活性 与整合素α6β1的结合活性,解离常数在10 nM以下。 laminin-511干细胞基质胶...
Laminin 511 E8 (LN511-E8) is a recombinant human protein that provides a defined surface for in vitro feeder-free culture of multiple human pluripotent stem cells (PSCs). Being a truncated form of laminin 511, LN511-E8 serves as a functionally minimal form that retain the full capability ...
511E8 has been reported by many as a superior culture substrate. However, the effects of 511E8 on human skin cells remain unclear. In this study, we added 511E8 during the culture period of a reconstituted skin equivalent (SE) and investigated its effect on the formation of BM-like ...
本制品是CHO-S细胞(Life Technologies)表达的重组人层粘连蛋白511-E8片段。 保存和稳定性 该产品可在2～15℃条件下稳定保存至标签上标示的有效期限内。避光保存。 iMatrix-511在4℃下稳定。 该产品的有效期为自制造日期开始2年。 活性 与整合素α6β1的结合活性,解离常数在10 nM以下。 laminin-511干细胞基质胶...
培养基质在干细胞培养中有重要的作用,如维持干细胞性,维持正常核型,维持表达干细胞标志物等。作为一款重组型的laminin-511 E8片段,iMatrix-511是一种改进型的干细胞培养基质。iMatrix-511提供了一种xeno-free的培养环境,并且允许在不含饲养层的情况下使用。另外,iMatrix-511相比较全长型laminin、vitronectin、Matrige...
In the development of cell culture systems, a fragment of laminin-511 (LM511-E8) was found to improve the proliferation of stem cells. The adhesion of undifferentiated cells to LM511-E8 is mainly mediated through integrin α6β1. However, the involvement of non-integrin receptors remains ...