The Binding activity of Laminin 511 E8 Fragment against ES cell was better than Full length 511 and traditional substrate. The same function was confirmed in human iPS cells. Fig.3. Dose-response adhesion curves of iPS cells on ECMS
Introduction Laminin 511 (LM511), a component of the skin basement membrane (BM), is known to enhance the adhesion of some cell types and it has been reported to affect cell behavior. A recombinant fragment consisting of the integrin recognition site; E8 region of LM511 (511E8) has also ...
In the development of cell culture systems, a fragment of laminin-511 (LM511-E8) was found to improve the proliferation of stem cells. The adhesion of undifferentiated cells to LM511-E8 is mainly mediated through integrin α6β1. However, the involvement of non-integrin receptors remains ...
Early studies with a proteolytically derived fragment oflaminin 111(fragment E8) recognized that secondary andtertiary structuresof the rod and LG1 to LG3 domains were necessary and sufficient for cell binding to the laminin C-terminus (Deutzmann et al., 1990). This structural requirement has bee...
Other investigators have reported that recombinant vitronectin12, a hydrogel13, and a LN-511 fragment14 can serve as alternatives to Matrigel or feeder cells for culturing of hES cells. However, none of the cell culture substrata allows clonal survival of hES cells without the use of apoptosis ...
On a molar basis B1B2-heterodimers were 24 times less efficient than PFHR9 laminin or EHS laminin in supporting cell attachment. These data suggest that heterotrimeric configuration is essential to the adhesive function of the laminin E8 fragment....
In the development of cell culture systems, a fragment of laminin-511 (LM511-E8) was found to improve the proliferation of stem cells. The adhesion of undifferentiated cells to LM511-E8 is mainly mediated through integrin 伪6尾1. However, the involvement of non-integrin receptors remains ...
5/LG1-3 contain an acidic residue required for integrin binding that is as critical as the Glu residue in the gamma 1 tail; to achieve this, we undertook exhaustive alanine substitutions of the 54 acidic residues present in alpha 5/LG1-3 of the E8 fragment of laminin-511 (LM511E8). ...
Deutzmann R , Aumailley M , Wiedemann H , Pysny W , Timpl R and Edgar D ( 1990 ) Cell adhesion, spreading and neurite stimulation by laminin fragment E8 depends on maintenance of secondary and tertiary structure in its rod and globular domain . Eur J Biochem , 191 , 513 – 522 ....
and intrinsic flexibility of integrin ectodomain. We have applied several unique methodologies to overcome these hurdles and solved the structure of the integrin α6β1 headpiece in complex with the E8 fragment of LM511 by cryo-EM, which is rapidly growing as a critical method for integrin struct...