L. Guarente, Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast, Methods Enzymol. 101 (1983) 181-191.Guarente L (1983) Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast. Methods Enzymol 101:181-191...
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In order to determine the β-galactosidase activity toward lactose as a natural substrate, the reaction assay was performed as described by [2] with minor modification. Briefly, 500 μl of a lactose stock solution (100 mM) was added to 480 μL of 50 mM of McIlvaine buffer (pH...
S Malarkannan,LM Mendoza,N Shastri - 《Methods in Molecular Biology》 被引量: 48发表: 2001年 T-cell antigen discovery (T-CAD) assay: a novel technique for identifying T cell epitopes A colorimetric assay and ovalbumin specific, lacZ inducible, T cell hybridomas were used to validate the sy...
These authors further showed that RLR1 (THO2; Piruat and Aguilera, 1998) and HPR1 (Chavez and Aguilera, 1997) are specifically required for transcriptional elongation by PolII through lacZ in yeast. We show that, in addition to its novel effect on lacZ transcription, rlr1 mutations cause ...
coli lacZ gene as a reporter of gene expression in C. glabrata was investigated. C. glabrata/ E. co... A Elbarkani,K Haynes,M Frosch - 《Gene》 被引量: 27发表: 2000年 Qualitative Assay for β-Galactosidase in Yeast Colonies This chapter illustrates the use of β -Galactosidase ( lacZ...
Explain Genetic Testing Assay Reports? Give examples. If you set up a positive control and it does not work, what does this tell you about your experiment? Explain. Discuss the importance of the 3d nuclear architecture in the regulation of gene expression. Explain the application of the conce...
半乳糖酶基因 ③ 大肠杆菌β -半乳糖酶基因(lacZ)的启动子及其编码的α - 肽链的 DNA 序列,此结构称 lac Z' 基因;④ 位于 lacZ' 基因中 … course.szu.edu.cn|基于135个网页 3. 半乳糖苷酸基因 ...列)有含有一个大肠杆菌DNA的短区段,其中含有β-半乳糖苷酸基因(lacZ)的调控序列和头146个氨基酸偏码...
9 RegisterLog in Sign up with one click: Facebook Twitter Google Share on Facebook lac operon (redirected fromLacz) Medical lac operon [′lak ′äp·ə‚rän] (genetics) Three adjacent linked genes which code for the enzymes that act sequentially in lactose utilization in many bacteria...
酵母双杂交,Yeasttwo hybrid(Y2H), 这是一个古老的技术,Stanley Fields 和 Ok-KyuSong 在 1989 年的时候利用大肠杆菌中 GAL4 乳糖操纵子的原理,开发出这个方法用于检测蛋白质之间的相互作用 。 GAL4 操纵子有两个结构域,BD(binding domain)结构域和 AD(activation domain),其中 BD 结构域可以结合在 UAS(upstr...