protoplasttransactivation assay. As for the yeast system, the cDNA encoding the candidate TF was subcloned into the yeastexpression vectorYepGAP and the resulting plasmid was introduced into the respective yeast reporter strain that had been used for TF screening. If the TF is able to activateHIS...
Human tumor-derived p53 proteins exhibit binding site selectivity and temperature sensitivity for transactivation in a yeast-based assay. Oncogene 1998, 16, 2527-2539. [CrossRef] [PubMed]Human tumor-derived p53 proteins exhibit binding site selectivity and temperature sensitivity for transactivation in ...
as shown by an overlay binding assay, also with an 18-kDa ribosomal or ribosome-associated protein from plants and yeast(Fütterer and Hohn, 1996). It also interacts with the 60S ribosomalsubunit proteinL18 (RPL18) fromArabidopsis thaliana(Lehet al., 2000), the interacting region being defined...
The identified HBx-interacting proteins that were possible to be involved in the HBV virus life cycle. Full size image Verification of the interaction between HBx and Pin1 The interaction between Pin1 and HBx was first confirmed using Ni2+-NTA pull-down assay. His6-tagged Pin1 and Flag-tagg...
and IPI7 with the GAL4 activation domain to form AD-IPI7. Blue clones in an X-Gal assay and clones grown on the SD-L-T-U medium indicate protein interaction in yeast cells.bGST pull-down assay for interaction between IPI7 and IPA1. MBP-IPI7, but not MBP, was pulled down with GS...
and IPI7 with the GAL4 activation domain to form AD-IPI7. Blue clones in an X-Gal assay and clones grown on the SD-L-T-U medium indicate protein interaction in yeast cells.bGST pull-down assay for interaction between IPI7 and IPA1. MBP-IPI7, but not MBP, was pulled down with GS...
NRL residues 1 to 57 were unable totransactivate in the yeast assay.These resultssuggest that NRL s minimal transactivation domain is likelyto encompass amino acids 30 to 93. The smallest minimal domainis potentially from residues 58 to 75. Determination of NRL sminimal domain can provide a ...
Transactivation activity assay of GhRAV1 protein in yeast cells.XiaoJie, LiMo, LiYing, ZhouShan, HuRong, HuYun, ChenXueBao, Li
Direct protein-protein interactions between Phd or PhLOP1 and CRX were demonstrated using a 尾-galactosidase quantitative assay in the yeast two-hybrid system and were confirmed by an in vitro binding assay and a glutathione S-transferase (GST) pull-down assay. To determine if the interaction ...
In vivo interaction between SmCAR and SmRXR1, SmRXR2 When AH109 yeast cells were co-transformed with BD-SmCAR and AD-SmRXR1, cells grew on −Leu, −Trp, −His, −Ade selective medium containing 2.5 mM 3-AT. The LacZ filter-lift assay was positive. However, yeast cells co-tra...