Shin T,Murao S,Matsumura E.A chromogenic oxidative coupling reaction of laccase: applications for laccase and angiotensin I converting enzyme assay. Analytical Biochemistry . 1987SHIN, T., MURAO, S., MATSUMURA, E. 1987. A chromogenic oxidative coupling reaction of laccase: application for laccase...
Thus, we developed a simple decolorization assay to assess the ability of our engineered laccases to degrade dyes and, by relation, toxic organic pollutants. Initially, we tested the ability of a commercial T. versicolor laccase (WT enzyme) to decolorize a selection of dyes responsible for ...
The promiscuity of these laccases for various aromatic compounds and their consideration as a “green” enzyme (i.e. one that produces water as the main by-product) explains why they have been extensively studied for their use in applications such as bioremediation, pulp and paper processing, ...
The present study performed laccase enzyme activity assay along with relevant biochemical analysis of developing cotton fib- ers to correlate laccase activity with biochemical analysis. The total soluble and wall bound phenolic compounds showed high abundance at 10 DPA followed by a steady decrease ...
The addition of Cu led to great increases in the laccase activity for G304K than for the wild-type enzyme, implying that the mutant had better stronger Cu-binding ability than that of the wild-type CueO (Fig. S6). The assay results were consistent with the results of structure analysis,...
niger different plate enzyme activity assays showed that all genes code for active multicopper oxidases, with apparent different degrees of affinity and specificity. The use of the A. niger GlaA secretion signal did not suppose an increase in McoA extracellular production and (NH4)2 tartrate ...
To study the effect of various metal ions on enzyme activity, the enzyme was incubated with (20 mM) HgCl2, FeSO4, AgNO3, MnSO4, MgSO4and ZnSO4at 40 °C for 10 min. Then, the enzyme assay was done by standard enzyme assay protocol. ...
Laccase-like nanozyme Nanoflowers 5-Hydroxyindole-3-acetic acid assay Laccase Amperometric sensor 1. Introduction 5-Hydroxyindoleacetic acid (5-HIAA) is an essential metabolite of the happiness hormone, serotonin which provides important information on physiological and pathological conditions within the hum...
For quantification of protein yield, samples of the NS loaded with the enzyme were first incubated in SDS 10% to denature the proteins and disassemble the nanospheres. Total protein content was measured using the DC Protein Assay Kit (Bio-Rad), following the manufacturer’s instructions. Then, ...
The invention relates to a new laccase enzyme, which can be isolated from the strains of the Melanocarpus genus, the M. albomyces strain in particular. The pH optimum