(https://www.biosino.org/node/project/detail/OEP002852). The CPTAC-HCC proteome data are available at the Proteomic Data Commons (PDC,https://pdc.cancer.gov/pdc/; accession node: PDC000198). Gene sets are available in Msigdb database (http://www.gsea-msigdb.org/gsea/msigdb/; C2 CP ...
In the case of macrophage histone H3, lactylation competes with acetylation at lysine residues to regulate the expression of a specific gene set. Extrapolating these findings to histone H1 and considering that histone H1 acetylation regulates chromatin compaction and decondensation and hence the ...
A Expression of genes within the lactylation gene set across cells; B scoring of each cell based on the lactylation gene set using the R package GSVA, scoring the level of the gene set across cell types; C lactylation gene set scoring as shown by UMAP (high and low level groups ...
Intriguingly, a notable loss of gene copy numbers suggests potential implications in tumor progression. Utilizing unsupervised clustering, KIRC samples were grouped into two distinct subcategories, each showcasing different survival outcomes. While pathway enrichment highlighted an aggressive, inflammation-...
Based on TCGA database, the enriched pathways activated and inhibited by RHOF were analyzed using Gene Set Enrichment Analysis (GSEA). Target binding between c-Myc and PKM2 promoters was predicted using MOTIF software. Human specimens This study was approved by the Ethics Committee of our ...
Given that HBO1 can also catalyze histone acetylation, particularly H3K14ac, we performed a set of comparative experiments to further verify the Kla-dependent gene regulation. Lactic acid (LA) has been considered as the precursor source of lactyl-CoA, which could be transferred to substrates by...
Similar to other epigenetic mark, the role of histone Kla was first described that can directly stimulate gene expression from chromatin [23]. Currently, histone Kla has been observed in many types of cells, predominantly in tumor cells and innate immune cells. In addition to histones, mounting...
To investigate the potential target genes regulated by H3K18la in senescent microglia, a total of 11,796 target gene of different H3K18la binding peaks at the promoter in senescent microglia were classified into different KEGG pathways. These KEGG pathways include the chemokine signaling pathway, ce...
The results of the gene knockdown and enzyme activity inhibition experiments all showed that lactate plays an important role in hypoxia-mediated aggravation of LPS-induced neuroinflammation in microglia. Previous studies have evaluated the impact of lactate on the inflammatory response in tumor-associated...
Collectively, our results suggest that an endogenous ‘lactate clock’ in bacterially challenged M1 macrophages turns on gene expression to promote homeostasis. Histone lactylation thus represents an opportunity to improve our understanding of the functions of lactate and its role in diverse ...