The present invention provides compositions and methods for detecting incorporation of a labeled nucleotide triphosphate onto the growing end of a primer nucleic acid molecule. The method is used, for example, to genotype and sequence a nucleic acid. In a preferred embodiment, the method described ...
生物工程专业英语翻译,帮忙翻译一下吧.拜托啦、2.8. RNase H assayIn a 10 Al volume, 1 nM a-32P-labeled uidA RNAtranscript, 50 nM oligodesoxynucleotide, 40 mM Tris–HCl pH 7.5, 4 mM MgCl2, 1 mM DTT and 5 unitsRNase H were mixed and
-phosphate of the nucleotide, will bereleased into the nanopore one at a time where they will produce unique current blockadesignatures. For the purpose of illustration only, several tags are shown inside the porein the order of their sequential release; in actuality only one tag will enter ...
Fig. 1: Electron potential maps and ribbon diagrams of pyrenyl-actin filaments with (a, c) bound Mg-ADP-Pi or (b, d) bound Mg-ADP. a, b Maps with highlighted densities of the nucleotide (turquoise) and pyrene (magenta) in one subunit (plum). Neighboring subunits are gray. The barbed...
(iii) a third nucleic acid that comprises a nucleotide sequence that is complementary to the target nucleic acid, which nucleotide sequence at least partially overlaps with the nucleotide sequence of the first nucleic acid primer; and (b) detecting the presence of catalytic nucleic acid activity, ...
Thermodynamics Of The Myosin Nucleotide-binding Pocket Measured By Epr Spectroscopy Using Spin Labeled NucleotidesNot Availabledoi:10.1016/j.bpj.2008.12.1076Purcell, Thomas J.Naber, NarimanPate, EdCooke, RogerElsevier Inc.Biophysical Journal
Finally, nine constructs hold insertions in the linker between the S6 transmembrane segment and the cyclic-nucleotide binding domain (CNBD), five insertions were in the CNBD, and the remaining twenty-three were in the remaining COOH terminus of the HERG sequence. This means that 48% of the ...
the cargo agent is an agent that finds use in contraception. In some embodiments, the therapeutic agent is selected from the group consisting of small molecules (e.g., nucleotide, steroid or other organic compound), polynucleotides, a lipid and a polypeptide (e.g., antibody or antibody fragm...
生物工程专业英语翻译,帮忙翻译一下吧.拜托啦、2.8. RNase H assayIn a 10 Al volume, 1 nM a-32P-labeled uidA RNAtranscript, 50 nM oligodesoxynucleotide, 40 mM Tris–HCl pH 7.5, 4 mM MgCl2, 1 mM DTT and 5 unitsRNase H were mixed and
Kits of the invention comprise a mutant DNA polymerase of the invention and a fluorescently labeled nucleotide that exhibits reduced discrimination with respect to the mutant DNA polymerase in the kit.ブランディスジョンブルームカーチスリチャーズ...