We selected an aptamer against a fluorogenic dye called Thioflavin T (ThT). Aptamers are single-stranded DNA that can bind a specific target. We selected the ThT aptamer using graphene oxide assisted SELEX and a low-cost Open qPCR instrument. We optimize
Secondly, most existing works relied on a limited amount of data from a single source (a single database or dataset generated from a single experiment), failing to fully take advantage of the available epi-transcriptome information. For example, the wide occurrence of m5U modification has been ...
To better understand the difference of digestive juice protein components in silkworm susceptible and resistant strains, a quantitative strategy was employed to identify proteins according to mass spectrum data. A total of 98 proteins, 89 of which with least 3 unique peptides, were identified in fou...
Several proteins, including the known BACE1 substrates APP, APLP1, CHL1 and contactin-2 showed lower abundance in the CSF of BACE1−/− mice, demonstrating that BACE1 substrate identification is possible from CSF. Additionally, ectonucleotide pyrophosphatase 5 was identified as a novel BACE1 ...
QFPD contains 21 herbal components optimized for the symptoms of COVID-19. Recent network pharmacological studies identified a large number of active natural compounds contained in the herbal ingredients of QFPD, and predicted the comprehensive molecular, biological and functional networks underlying its...
Maize is one of the most important food crops worldwide. Roots play important role in maize productivity through water and nutrient uptake from the soil. Improving maize root traits for efficient water uptake will help to optimize irrigation and contribu
Citrus is one of the most important and widely grown commodity fruit crops. In this study a label-free LC-MS/MS based shot-gun proteomics approach was taken to explore three main stages of citrus fruit development. These approaches were used to identify
MicroRNAs (miRNAs) bind to the 3ʹ-untranslated region of target mRNAs in a sequence-specific manner and subsequently repress gene translation. Human miR-26a has been studied extensively, but the target transcripts are far from complete. We first emplo
Consequently, the retention of nuclear DNA label has been used as a measurement of slow proliferation rate to identify potential tissue-specific stem cells termed the label-retaining cells (LRCs)11. Prior to the era of advanced mouse genetics a pulse administration of nucleotide analogues such as...
Here we propose a new predictor called “pLoc-mEuk” by extracting the key GO (Gene Ontology) information into the general PseAAC (Pseudo Amino Acid Composition). Rigorous cross-validations on a high-quality and stringent benchmark dataset have indicated that the proposed pLoc-mEuk predictor is...