Label-free imaging of mobile dynamin oligomers Raw mass photometry images of dynamin on an SLB exhibited an optical background caused by the roughness of the microscope coverslip (Fig. 1a; raw images). By implementing a sliding median background subtraction33, we obtained a nearly shot noise-lim...
The detection limit of LINA depends on several factors, notably the size distribution of the training dataset and the limitations in the images resulting from the microscope (contrast, SNR, resolution) and the sample (transparency, refractive index heterogeneity). In our training dataset, we focused...
tree based on the 2D 8-connected work of Wu, Otoo, and Suzuki. This implementation is compatible with images containing many different labels, not just binary images. It also supports continuously valued images such as grayscale microscope images with an algorithm that joins together nearby values...
The numbers of markers used for training (70%) and testing (30%) are indicated for each compartment. F1 scores are the harmonic mean of recall (true positives / [true positives + false negatives]) and precision (true positives / [true positives + false positives]) based on the test set...
The mechanical, thermal, chemical, physical, water absorption and morphological properties of Borassus flabellifer fibers have also discussed. The researchers have used the Borassus flabellifer fruit fibers, leaf stalk fibers, petiole fibers respectively for composite fabrication. All the parts of the ...
Such CASA systems comprise a lens-based microscope with a digital camera connected to a PC used for capturing sequential frames. These digital images are subsequently processed using custom designed software to detect and track the heads of the sperms9. Using conventional lens-based microscopes, ...
Both the energy metabolism of the cell components and the structuring of the collagen fibrils were addressed. Results Based on the data from the fluorescence lifetimes and the contributions of protein-bound NAD(P)H, a bias toward oxidative metabolism was indicated, for the first time, in both ...
A compact microplate imaging system, including: a tunable light source; a lens ensemble to collimate the light source onto the microplate and to transmit light that is reflected from the microplate; a
performed by a single technician using simple touchscreen instructions. A freshly excised surgical specimen is loaded directly into a custom microscope slide and inserted into the SRH imager without the need for tissue processing. Additional details on image acquisition are provided in Extended Data Fi...
parts of the chip, filter pillars were implemented and their design improved11. We introduce multiple columns of increasingly narrowly spaced filter pillars, allowing the successive retention or resolution of interfering objects. The distance between pillars at the first column is 60 µm (indicated ...