The bound antibodies were visualized using diaminobenzene, counterstained and mounted as described before. Image acquisition of tissue sections Sections that were stained for insulin and the GLP-1 receptor were scanned with an automated microscope (Pannoramic 1000 (P1000)) using an objective ...
Proteins from the prismatic calcite layers accumulate at the edge of crystals while proteins from the nacreous aragonite layers accumulate at the center of crystals. Raman and X-ray powder diffraction show that both the proteins cannot alter the crystal phase. Scanning electron microscope demonstrates ...
Lysis was monitored by light microscope. Larger particles were removed by centrifugation (10 min at 600 g) and the supernatant was passed through a 0.2-μm filter. Protein contents were determined and aliquots were stored at -80°C until use. Treatment Patients were generally vaccinated ...
The interaction of a near infrared (NIR) laser beam with cells has been addressed from many different view angles. From a macroscopic perspective, low levels of NIR laser light are used to reduce pain, inflammation, nerve injuries and to promote tissue regeneration10,11,12. Studies of the inf...
Quarles, R.H., Brady, R.O.: Synthesis of glycoproteins and gangliosides in developing rat brain. J. Neurochem. 18, 1806–1820 (1971) Google Scholar Rambourg, A., Leblond, C.P.: Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cells in the ...
Scanning electron microscope demonstrates both proteins are able to affect the crystal morphology. This study may provide a direct approach for the visualization of protein distributions in crystals by small-molecule dye-labelled proteins as the additives in the crystallization process and improve our ...
The fluorescence imaging was performed with an inverted microscope (Olympus IX51, Tokyo, Japan) using an excitation filter BP460-490, beam splitter DM500 and a barrier filter BA520IF. The intensity of fluorescence emission was captured using a CCD DP71-1-5 camera (Olympus, Tokyo, Japan). ...