When he went to college in Paris, he showed interest in looking through a microscope. A microscope makes things look a lot bigger; it lets you see things you can’t see just with your eyes. Looking through a microscope, Pasteur found a living world in a drop of water. He saw and ...
One day a winemaker came into the lab hoping that someone could help him with his problem. “Sometimes my wine tastes delicious but sometimes it turns sour(酸的)” he said to Pasteur. “Can you help me find out why?” Pasteur put some drops of the wine under the microscope. He noticed...
Label-free imaging of mobile dynamin oligomers Raw mass photometry images of dynamin on an SLB exhibited an optical background caused by the roughness of the microscope coverslip (Fig. 1a; raw images). By implementing a sliding median background subtraction33, we obtained a nearly shot noise-lim...
This prism allows the acquisition of an image stack consisting of eight z-planes simultaneously. An algorithm is then used to retrieve phase information from the brightfield image stack using Fourier filtering, transforming it into a QPI stack. We used this microscope to collect a dataset of ...
aThe thixoformed parts and the slugs quenched in water from the reheating temperature were sectioned and were prepared with standard metallographic practices and finally etched with a 0.5% HF solution before they were examined with an optical microscope. Both dark field and bright field imaging techn...
Raman scattering-based technique coupled with microscope provides information about the chemical structure of biological specimen at the molecular and cellular scale nondestructively without the requirement of chemical extraction. Confocal Raman microspectroscopy presents a new approach for investigating the ...
A frameless stereotaxic operating microscope for neurosurgery. Projected images superpose the surgical field, reconstructed from image data to match the focal plane of the operating microscope. The algorithms on which ... E,M,Friets,... - 《IEEE Transactions on Bio Medical Engineering》...
aSamples were incubated with 5 mg mL1 Phalloidin-FITC (green, Sigma-Aldrich) and 5 mg mL1 Hoechst 33258 (blue, Sigma-Aldrich) in culturemediumfor 20minutes at 37 C, then fixed with 3.7%paraformaldehyde for 30minutes and imaged under a Leica TCS-SP2 Confocal Microscope using TCS Leica So ...
performed by a single technician using simple touchscreen instructions. A freshly excised surgical specimen is loaded directly into a custom microscope slide and inserted into the SRH imager without the need for tissue processing. Additional details on image acquisition are provided in Extended Data Fi...
(CellVista SLIM Pro, Phi Optics, Inc.), which allows for fully automated data acquisition. The microscope objective pupil is relayed onto the surface of a phase-only spatial-light modulator (SLM), such that the phase shift between the incident and scattered light is controlled precisely (Fig....