Microscope Parts & SpecificationsHistorians credit the invention of the compound microscope to the Dutch spectacle maker, Zacharias Janssen, around the year 1590 (more history here). The compound microscope uses lenses and light to enlarge the image and is also called an optical or light microscope...
An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. It is a special type of microscope having a high resolution of images, able to magnify objects in nanometres, which are formed by controlled use of electrons in a vacuum captured ...
Grids were frozen by plunging into liquid ethane cooled to about −180 °C and screened on a Talos electron microscope operated at 120 kV and equipped with a Ceta 16 M camera (FEI company, Hillsboro, OR). The two datasets were collected in a consecutive session on a Titan Krios ...
PBS-washed cells were incubated with 4,6-diamidino-2-phenylindole (DAPI; Invitrogen, CA, USA) to stain the nucleus for 10 min, and the cells were visualized with a LSM880 confocal microscope (Carl Zeiss, Jena, Germany). Molecular colonoscopic imaging of orthotopic colon cancer mice Before ...
axarquiensis TUBP1 and labeled a green fluorescence by electroporation, which didn't change the bioactivity in vitro. In gnotobiotic conditions, cotton seeds were then inoculated with the gfp -labeled strain and grown in green house. Observation with a confocal laser scanning microscope and a ...
Visualization was performed with a fluorescent microscope Zeiss Axio Scope.A1 (Zeiss, Oberkochen, Germany) and fluorescence microscope (Nikon, Japan). The RGC were counted manually using ImageJ software with Cell Counter plugin (http://imagej.nih.gov/ij/). For cell counting purpose, from each ...
A Zeiss Axiovert 100 microscope with a 40×/0.75 Plan-NeoFluar objective (Carl Zeiss) was used. The fluorescence imaging system (Till-Photonics, Gräfelfing, Germany) consisted of a monochromator Polychrome IV, a dual band CFP/YFP filter set and a 12-bit CCD camera (IMAGO) combined with ...
The slide images were digitalized using a microscope (Axio Imager.Z2, Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany) and cropped to a size of 13,020 × 10,422 px2, resulting in images with a pixel size of 0.5 µm. 2.2. Data Preparation To reduce the network’s complexity...
Images were recorded with a fluorescent microscope (DM 6000 B, Leica, Wetzlar, Germany). Molecules 2016, 21, 1144 17 of 23 4.8.1. Inhibition Assay EA.hy926 cells were seeded in culture medium at confluency the day prior to experiment or in case of the Caco-2 cells two days prior to ...
After 2 h, the applied substances were removed from the medium and washed three times with PBS. Lastly, 2 µL/mL DAPI was added, and the related cell images were taken using a fluorescent microscope with a fluorescent microscope (Olympus BX53) 10× green filter. At the same time, cell...