www.nature.com/scientificreports OPEN Direct label-free measurement of the distribution of small molecular weight compound inside thick received: 01 April 2015 biological tissue using coherentaccepted: 07August 2015 Raman microspectroscopyPublished: 10 September 2015 Masahiko Kawagishi1, Yuki Obara2,...
We describe a new oblique-incidence reflectivity difference (OI-RD) scanning microscope for high-throughput screening, in microarray format on functionalized glass slides, small-molecule compound libraries for protein ligands. The microscope employs a combination of scan mirror for y-scan and single-...
The surface morphology analysis can be carried out by Scanning Electron Microscope (SEM). After alkali treatment, the surface morphology of fibers has shown the significant changes occurred on the surface of Borassus fruit fibers. The fiber surfaces have gold coated before recording the micrographs (...
Osteoarthritis (OA) is characterized by the progressive deterioration of articular cartilage, involving complicated cell-matrix interactions. Systematic investigations of dynamic cellular and matrix changes during OA progression are lacking. In this study, we use label-free two-photon excited fluorescence (...
A compact microplate imaging system, including: a tunable light source; a lens ensemble to collimate the light source onto the microplate and to transmit light that is reflected from the microplate; a
The average of 10 spectra is presented for each compound. dividing cells, F1 is spread across the entire cell except for the nucleus, suggesting that it is due to ordi- nary cytoplasmic autofluorescence. In stark contrast, F2 shows intense signal predominantly in dividing cells (see regions ...
Discovery of fatty-acid-accumulated mitochondria in living cancer cells during hypothermia exposure. (A) Schematic of the CARS/MPEF hybrid microscope. (B) Illustration of denoising for hyperspectral and time-lapse image stacks. (C) An original CARS image at CH2 vibration frequency from MIAPaCa-2...
Water and lipids are key participants in many biological processes, but there are few non-invasive methods that provide quantification of these components in vivo, and none that can isolate and quantify lipids in the blood. Here we develop a new imaging
We reveal a novel design for dye-doped liquid crystal (DDLC) microfluidic biosensing chips in the polydimethylsiloxane material. With this design chip, the orientation of DDLCs was affected by the interface between the walls of the channels and DDLCs. Wh
reflects the excitation light into a beam expanding telescope (L5, L6), whereas a broadband mirror (M) placed at 45 degrees is used to guide the laser beam into a modified inverted microscope serving as the platform of the hybrid imaging system. An objective lens (OL; Achromat 8X, LOMO, ...