Label-free quantitativeproteomics(Labelfree)technology 概述: 定量蛋白质组学是蛋白质组研究的重要内容,通过对基因表达产物——蛋白质的定量分析,以了解基因在不同的生理、病理和逆境条件下的表达情况。近年来,随着非凝胶技术的发展,“鸟枪法”蛋白质组学(“Shotgun”proteomics)技术,特别是多维蛋白质鉴定(Multidimension...
Label-free quantification is a method of determining the relative amount of proteins in two or more biological samples, but unlike other quantitative methods, it does not use a stable isotope for chemical binding and labeling of proteins. Label-free quantitative proteomics approach provides a ...
Label-freeQuantitativeProteomicsAnalysisofaWheatNear-isogenicLinePair 系统标签: exactive锈病小麦标记isogenic基因 ·研究报告· 生物技术通报 BIOTECHNOLOGYBULLETIN2014年第6期 小麦条锈病是由小麦条锈病菌(Puccinia striiformisf.sp.tritici)引起的小麦叶部病害,严重时 也危害穗部。小麦条锈病在许多国家和地区均有发 生...
lactobionicantibacterialmultitargetedproteomicsmethicillinresistant Label-FreeQuantitativeProteomicsRevealstheMultitargetedAntibacterialMechanismsofLactobionicAcidagainstMethicillin-ResistantStaphylococcusaureus(MRSA)usingSWATH-MSTechnologyShimoKang,FanhuaKong,XiaonaLiang,MohanLi,NingYang,XueyanCao,MeiYang,DongbingTao,XiqingYue,...
Quantitative proteomics is key for basic research, but needs improvements to satisfy an increasing demand for large sample series in diagnostics, academia and industry. A switch from nanoflowrate to microflowrate chromatography can improve throughput and reduce costs. However, concerns about undersamplin...
Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology Abstract MicroRNAs (miRNAs) bind to the 3ʹ-untranslated region of target mRNAs in a sequence-specific manner and subsequently repress gene translation. Human miR-26a has been...
Label-free quantitative proteomics analysis models in vivo and in vitro reveal key proteins and potential roles in sciatic nerve injury Background:The underlying mechanism of sciatic nerve injury(SNI)is a common motor functional disorder,necessitates further research.Methods:A rat model of ... GU ...
For peptides the maximum false discovery rate (FDR) was set to 0.01 and 0.05 for proteins. A two minute window for match between runs was set when label free quantification was enabled. Each file was kept separate in the MaxQuant experimental design template, to obtain individual quantitative...
To identify novel substrates of E3s that are targeted for degradation, we set up a global strategy using label-free quantitative proteomics with cells induced to express either a functional or a defective E3 ligase (Fig. 1). To evaluate this method, expression vectors encoding FLAG-tagged ASB2...
Then we performed label-free quantitative proteomics combined with liquid chromatograph–mass spectrometer/mass spectrometer (LC-2D-MS/MS）analysis and bioinformatics analysis to find the potential biomarkers for VC. In the current study, we identified 113 significantly proteins. Fifty...