Label-free quantification (LFQ)Mass spectrometry (MS)Quantitative proteomics analysisTriticum aestivumTo distinguish protein abundance changes in biological systems under different conditions, mass spectrometry-based proteomics provides a powerful tool to detect and quantify such responses. Improvements in mass...
Label-free quantification is a method of determining the relative amount of proteins in two or more biological samples, but unlike other quantitative methods, it does not use a stable isotope for chemical binding and labeling of proteins. Label-free quantitative proteomics approach provides a ...
On the other hand, the changes of tea proteomics in this process were analyzed by the label-free quantification technology, and the key proteins that can derive these potential antioxidant peptides were preliminarily selected. An insight of the changes of tea proteomics in fermentation and its ...
Label-free Mass Spectrometry proteomic quantification was undertaken commercially by Functional Genomics Centre Zurich (ETH Zurich, University of Zurich, Switzerland). The isolated proteins were solubilized using High Intensity Focused Ultrasound (HIFU) for 1 minute. The ultrasonic amplitude for HIFU was...
1) Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ. 2) Mass Spectrometry-Based Label-Free Quantitative Proteomics. 3) A label-free differential proteomics analysis reveals the effect of melatonin on promoting fruit ripening and...
Clinical proteomics and systems biology studies require high-throughput liquid chromatography with tandem MS (LC–MS/MS) analyses with deep proteome coverage and accurate quantification. To achieve this, it is necessary to reduce MS measurement time by deploying shorter LC gradients with faster scanning...
Label-free quantificationColiform mastitis remains a primary focus of dairy cattle disease research due in part to the lack of efficacious treatment options for the deleterious side effects of exposure to LPS, including profound intra-mammary inflammation. To facilitate new veterinary drug approvals, ...
The workflow is validated by a comparative analysis of BACE1-/- and wild-type mice using label-free quantification. The protease BACE1 cleaves the amyloid precursor protein (APP) as well as several other substrates and is a major drug target in Alzheimer's disease. We identified a total of ...
The workflow is validated by a comparative analysis of BACE1−/− and wild-type mice using label-free quantification. The protease BACE1 cleaves the amyloid precursor protein (APP) as well as several other substrates and is a major drug target in Alzheimer's disease. We identified a total ...
Quantitative proteomics allows the quantification of the alterations between normal and disease samples, which provides useful information to identify novel biomarkers and elucidate mechanisms in complex biological processes and conditions [5]. To the best of our knowledge, however, no studies using ...