Kinase Reaction Buffer A 6-1721 10mL 995英文名称:Kinase Reaction Buffer A 运输:4-25℃ 保存:4-25℃ 有效期:1 Year 货期:2-4 days 其他: 产品介绍:收起 Order Online在线订购 订货信息 产品名称 * 申请类型 * 套数 * 推荐人 真实姓名 * 邮寄地址 * 所属单位 电子邮箱 * 联系电话 * 联系QQ ...
Kinase Reaction Buffer Concentrate, 60ML 供应商 Arbor Assays 产品货号 X104-60ML 产品报价 ￥询价/60ML 产品说明书 点击查看 购买方式 Arbor Assays中国区现货中心已经建立,90%产品中国有现货库存。银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868 ...
上海玉博生物科技有限公司 主要致力于“SER/THR KINASE REACTION BUFFER Invitrogen-KB001A”的生产销售。多年的“SER/THR KINASE REACTION BUFFER Invitrogen-KB001A”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用户信赖。欢
Reaction Buffer A(10X)(用于磷酸化反应):500mM Tris-HCl(pH7.6 at 25℃),100mM MgCl2,50mM DTT,1mM spermidine,1mM EDTA。 Reaction Buffer B(10X)(用于交换反应):500mM imidazole-HCl(pH6.4 at 25℃),0.18M MgCl2,50mM DTT, 1mM spermidine,1mM EDTA,1mM ADP。
The beads were then incubated with 100 μg of cell lysates in a final volume of 50 μl of kinase reaction buffer (10 μl of 5 × kinase buffer, 10 μl magnesium/ATP cocktail solution [90 μl of 75 mM MgCl2/500 mM ATP and 10 μl (100 μCi) [γ-32P]...
Briefly, 1 μg of bacterially purified GST-GSK-3β recombinant proteins were incubated with immunoprecipitated AKT1 from cells in kinase reaction buffer (1 μM MnCl2, 2 mM DTT, 50 mM Tris pH 7.5) for 30 min at 30 °C. The reaction was stopped by adding 3× SDS loading ...
Kinase Reaction Buffer Concentrate 60 mL Catalog Number X104-60ML A 2X concentrate containing detergents and stabilizers. Cell Lysis Buffer 100 mL Catalog Number X050-100ML A Tris based bu er containing detergents. Store Frozen as this bu er contains no preservatives Cell Lysis Buffer must be ...
Total kinase activity on Hsp27 was measured using 1 μl (2–3 μg) of cardiomyocyte lysate prepared as indicated above and 1 μg of recombinant Hsp27 in 20 μl of kinase reaction buffer containing 25 mM MOPS, 15 mM MgCl2, 25 mM β-glycerophosphate, 1 mM EGTA, 0.1 mM NaF, 4 mM ...
Note:1X Kinase Buffer A may be stored at room temperature for future use. Note:This section is taken directly from the Src validation packet, Step 3. Note:If your kinase requires additives such as calcium and calmodulin, please be sure...
After shear stress experiments, the cells were lysed in the kinase lysis buffer. Myc-ERK2 was immunoprecipitated with anti-c-Myc mAb and protein A-Sepharose beads, followed by Myc-ERK2 kinase activity assays using the same procedures as described above. The kinase activities of the endogenous ...