Theprotein kinasesarea kinasefamily member and regulate the mechanism of phosphorylation by transferring a phosphate group from ATP to the Ser/Thr or Tyr residues of a particular protein. Phosphorylation controls protein activity by triggering conformational changes or by interrupting and initiatingPPI(46...
calmodulin binding, which is, in turn, regulated by protein kinase C phosphorylation. The kinase has numerous protein substrates in the cell; however, the precise mechanism of cellular regulation is not fully understood. Sign in to download full-size image...
At a molecular level, the mechanism by which multiple phosphorylation events impact the Hsp90 chaperone cycle remains unexplored. We show that the kinase-specific cochaperone Cdc37 promotes tyrosine phosphorylation of Hsp90 in a client class-specific manner. Our data reveal a mechanism by which spec...
first, we decided to use an in vitro cardiomyocyte model. On the one hand, cardiomyocytes overexpressingClk4were resistant to phenylephrine (PE)-induced hypertrophy, which was rescued by simultaneous knockdown ofNexn. On the other hand, overexpression of a phosphorylation-mimic mutant of NEXN (S4...
induces conformational changes in the N-lobe of receiver kinase [9] which induces activation of the ‘receiver’ kinase domain andtrans-phosphorylation of tyrosine residues in the C-terminal tail of the ‘activator’. Phosphorylation of the activation loop is not involved in this mechanism [24,25...
that would keep PLD inactive when PKCζ is active. However, our data do not support this, since kinase inactive PKCζ or myristoylated PSζ did not stimulate PLD activity in the absence of PHE stimulation. Moreover, PLD phosphorylation is probably not involved in its mechanism of activation [...
Similar to FAK/p125FAK, also the MAP-kinases p42/44ERK and p38 stress-kinase revealed disconnection concerning modulation of protein quantity and phosphorylation/activation at the given strain periods. This finding shows on the one hand that modulation of intracellular signalling occurs uncoupled from...
chain kinase activation, Ca2+-induced Ca2+sensitization whereby Ca2+entry activates the RhoA/Rho-associated kinase pathway leading to phosphorylation of MYPT1 (the myosin targeting subunit of myosin light chain phosphatase) and inhibition of the phosphatase. Investigations into the mechanism of activation...
Hirano I, Nakamura S, Yokota D, Ono T, Shigeno K, Fujisawa S, Shinjo K, Ohnishi K (2009) Depletion of Pleckstrin homology domain leucine-rich repeat protein phosphatases 1 and 2 by Bcr-Abl promotes chronic myelogenous leukemia cell proliferation through continuous phosphorylation of AKT isoforms...
The [3H]-deoxythymidine phosphorylation assay was one of the earliest methods used to measure TK1 activity through radioactivity, thereby avoiding excessive use of reagents and increasing the number of samples available for processing [110]. For several years, this test was considered to be the “...