The protein kinases are a kinase family member and regulate the mechanism of phosphorylation by transferring a phosphate group from ATP to the Ser/Thr or Tyr residues of a particular protein. Phosphorylation controls protein activity by triggering conformational changes or by interrupting and initiating...
complex or to a combination of the two. Despite these limitations, assembling a chimeric model that contains our yeast DH and the human DDK structures35can further inform the phosphorylation mechanism. Here, the N-terminal end of the yeast Mcm4 model appears suitably aligned with the C-terminal...
The structures reveal an intrinsically active kinase conformation, with catalytic residues and a catalytic mechanism remarkably similar to canonical protein kinases. The active site is highly recessed owing to the FKBP12–rapamycin-binding (FRB) domain and an inhibitory helix protruding from the ...
calmodulin binding, which is, in turn, regulated by protein kinase C phosphorylation. The kinase has numerous protein substrates in the cell; however, the precise mechanism of cellular regulation is not fully understood. Sign in to download full-size image...
These results support the notion that CK1alpha is able to mediate GPCR phosphorylation in an agonist-dependent manner and that this may provide a novel mechanism for GPCR phosphorylation. The functional role of phosphorylation was investigated using a mutant of the m3-muscarinic receptor that showed ...
that would keep PLD inactive when PKCζ is active. However, our data do not support this, since kinase inactive PKCζ or myristoylated PSζ did not stimulate PLD activity in the absence of PHE stimulation. Moreover, PLD phosphorylation is probably not involved in its mechanism of activation [...
induces conformational changes in the N-lobe of receiver kinase [9] which induces activation of the ‘receiver’ kinase domain andtrans-phosphorylation of tyrosine residues in the C-terminal tail of the ‘activator’. Phosphorylation of the activation loop is not involved in this mechanism [24,25...
Here, we describe the mechanism by which suppressor of IKK epsilon (SIKE) inhibits TBK1-mediated phosphorylation of interferon regulatory factor 3 (IRF3), which is essential to type I interferon production. Kinetic analyses showed that SIKE not only inhibits IRF3 phosphorylation but is also a high...
The study of phosphorylase kinase (PhK)-regulated glycogen metabolism has contributed to the fundamental understanding of protein phosphorylation; however, the molecular mechanism of PhK remains poorly understood. Here we present the high-resolution cryo-electron microscopy structures of human muscle PhK. ...
Site-specific phosphorylation of Hsp90 has a differential impact on the maturation of different classes of clients, including protein kinases and transcription factors25,27,47. Our study suggests that the cochaperone-mediated recruitment of modifying enzymes provides a mechanism to generate highly specifi...