Gene SummaryThis gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multipl...
Kdm1a Gene ID 99982 品系名称 遗传背景 C57BL/6J 修饰方式 KO 应用领域 表型 该基因的纯合子缺失导致异常原肠胚形成和早期胚胎死亡。缺乏神经特异性亚型的纯合子是低兴奋性的,对药理学诱导的癫痫发作的敏感性降低。 品系状态 冷冻 目录号 VSM4004384 ...
病毒包装按照厂家说明书(addgene)进行,病毒质粒及包装质粒在293t细胞进行转染,转染48h后,收集上清,进行病毒滴度鉴定,分装后保存于-80℃冰箱。购买商品化的scrambleshrna(scramsh)作为kdm1ash的对照。kdm1ashrnas的目的序列为:5’-aaataaaggtttgactcgtgg-3’(seqidno:1)(2)病毒转染根尖牙乳头干细胞铺板过夜,慢...
表 1 引物信息 Table 1 Primer information 基因 Gene GAPDH 引物序列(5'-3') 退火温度 产物长度 Sequence of primer Tm(℃) Product size (bp) F: TGCTGGTGCTGAGTATGTGGTG 60 293 R: TCTTCTGGGTGGCAGTGATGG KDM1A F: CGCAAAGGAAACTATGTAG 60 245 R: ATTATTGAGGACGTTGAAGTC F:正向引物;R:反向引物...
Results: Deletion of the KDM1A/LSD1 gene occurred in 19 samples (38%), whereas ZNF217 gene amplification was identified in 11 samples (22%). We found a significant association between lymph node metastasis or advanced tumour stage and KDM1A/LSD1 gene deletion (p value=0.0003 and p value=...
1. Sen, G. L., Boxer, L. D., Webster, D. E., Bussat, R. T., Qu, K., Zarnegar, B. J., ... & Khavari, P. A. (2012). ZNF750 is a p63 target gene that induces KLF4 to drive terminal epidermal differentiation. Developmental cell, 22(3), 669-677. ...
1. Sen, G. L., Boxer, L. D., Webster, D. E., Bussat, R. T., Qu, K., Zarnegar, B. J., ... & Khavari, P. A. (2012). ZNF750 is a p63 target gene that induces KLF4 to drive terminal epidermal ...
KDM1A-mediated H3K4 demethylation is a well-established mechanism underlying transcriptional gene repression, but its role in gene activation is less clear. Here, we report a critical function and mechanism of action of KDM1A in glucocorticoid receptor (GR)-mediated gene transcription. Biochemical ...
保存条件4℃可保存3个月,-20℃可保存1年。 背景资料Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins.LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone protei...
Transposable elements (TEs) contribute to gene expression regulation by acting as cis-regulatory elements that attract transcription factors and epigenetic regulators. This research aims to explore the functional and clinical implications of transposable