differentcellmodelswereextracted,respectively.Then,theexpressionsof,Yglobingene ,fetalhemoglobin,p38,andthe levelofp38phosphorylationwereanalyzedbyRT?PCR,Westernblotting,andbenzidinest aining.Results:Theresultsof RT—PCRandWesternblottingshowedthattherewerenoevidentchangesinthemRNAan ...
Function of Normal and Mutated y-Globin Gene Promoters in Electroporated K562 Erythroleukemia CellsWe examined the importance of cis-acting regulatory elements of the human gamma-globin gene promoter in a cell line (K562) where this gene normally functions. A gamma-Globin promoter fragments were ...
andGγ-globininK562cells.MethodsK562cellsweretreatedwithAPSattheconcentrationof150,300,and450mg/L,with Na-buytrate(NaB)-treatedcellsservingasthepositivecontrolanduntreatedcellsastheblankcontrol.Benzidinestainingwas usedtoexaminethechangesinhemoglobinsynthesisinK562cellsafterthetreatments, ...
1)K562 cell lineK562细胞系 1.Methods The recombinated Ad-PTEN containing green fluorescent protein(GFP)was transfected into leukemia cells and K562 cell lines.目的探讨肿瘤抑制基因PTEN对人原代白血病细胞及K562细胞系的增殖、凋亡及细胞周期的影响。 英文短句/例句 1.The Effect and Molecular Mechanism for...
A potential strategy for treatment of sickle cell disease (SCD) and β-thalassemia in adults is reactivation of the ε- and γ-globin genes in the adult. We aimed to identify trans-activators of ε- and γ-globin expression and provide new candidate targets for effective treatment of sickle ...
4.Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China 通讯作者:方向东,博士,研究员,研究方向:干细胞与复杂疾病临床组学与转化医学。E-mail:fangxd@big.ac.cn 编委:杨昭庆 收稿日期:2018-06-29修回日期:2018-09-30网络出版日期:2018-11-20 ...
红系分化标志蛋白！-globin 、"—globin y -globin 表达增加，促进K562细胞向红系定向分化。关键词 6,7二甲氧基香豆素；滨蒿内酯；红系分化；新生儿溶血性贫血 中图分类号 R965 文献标志码 A 文章编号 1673-7806（2020）06-413-04 母儿血型不合是指孕妇与胎儿间血型不合产 生的同族血型免疫病叫该病可导致...
A beta-globin gene, inactive in the K562 leukemic cell, functions normally in a heterologous expression system. The K562 human leukemia cell is an erythroid-like cell that may serve as a model for the study of globin gene expression in transcriptionally active human ... C,M,Fordis,... -...
采用流式细胞仪(FACS)S1作用后K562细胞周期被抑制的情况;用Western-blotting方法检测不同浓度的S1作用后K562细胞中B细胞白血病2(B-cell leukemia-2,Bcl-2)蛋白的变化情况.结果提示:S1作用后的细胞与对照组相比,状态较差,说明细胞生长被抑制.S1与常规化疗药物阿糖胞苷联合应用,联合指数均小于1,提示二者具有协同的...
cellsisregulatedbyRabl1.JCellSci,2002.115(Pt12):2505— 2515. 12BianchiN,ChiarabelliC,BorgattiM,eta1.Accumulationofgain- ma-globinmRNAandinductionoferythroiddifferentiationafter treatmentofhumanleukaemicK562cellswithtallimustine.BrJ Haemato1.2001,l13(4):95 卜961. 13DaviesJH.EvansBA.JenneyME,eta...