Trans IT ®-Jurkat Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in Jurkat cells and cell types of associated lineage. Jurkat cells (human T-lymphocyte; acute T-cell leukemia cell line) have been inherently difficult to transfect, yet ...
原装正品|GenMute siRNA Transfection Reagent for Jurkat CellDescription:GenMute™ siRNA Transfection Reagent for Jurkat Cell is pre-optimized for transfecting siRNA to Jurkat cells. Jurkat cells are an immortalized line of T lymphocyte cells that are used to study acute T cell leukemia, T cell ...
Transfection:In each well of a 96-well plate, 150K log-phase Jurkat cells were seeded in 0.1mL of RPMI-1640 media 6 hours before transfection. Plasmid DNA (100ng) was diluted in 10μL of serum-free media. Turbo...
transfectionJurkat, an immortalized cell line derived from human leukemic T lymphocytes, has been employed as an excellent surrogate model of human primary T-cells for the advancement of T-cell biology and their applications in medicine. However, presumably due to its T-cell origin, Jurkat cells ...
Viable cells are clear but stain dark blue if nonviable. Transfection In a single transfection, 107 Jurkat cells are centrifuged at 100g for 5 min, the supernatant is discarded, and the cell pellet is resuspended in 250 μl of RPMI–10% (v/v) FCS (final concentration of 40 × 106 ...
Jurkat, an immortalized cell line derived from human leukemic T lymphocytes, has been employed as an excellent surrogate model of human primary T-cells for the advancement of T-cell biology and their applications in medicine. However, presumably due to its T-cell origin, Jurkat cells are very ...
Using Jurkat T-cells exposed to PMA and heat killed (HK) Escherichia coli MG1655 in combination with inhibitors of NF-κB, JNK and PKC, we demonstrated that NF-κB regulates IL-6 expression while the regulation of CXCL8 more closely correlated to AP-1 activity. These results indicate that ...
Lipofectamine® LTX 试剂采用专有的无动物源性配方,用于将 DNA 转染到真核细胞中,且具有低细胞毒性。本参考文件提供了使用 Lipofectamine® LTX 试剂(货号 15338-100)将质粒 DNA 转染到人 Jurkat 人 T 细胞白血病细胞(ATCC 货号 TIB-152)中的推荐程...
Jurkat (clone E6-1), human T lymphoblast, suspension Supplied As Each vial contains ˃1 x 106cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796) Materials Required But Not Supplied Media Required for Cell Culture NameOrdering Information ...
提前准备 jurkat 调节培养基: 从健康的、未经处理的 jurkat t 细胞中取出 rpmi w/ab 培养基, 生长到 1 x10 6 cells/mL ml, 离心剂 5分钟, 在 300 x克处, 并使用0.22 微米的注射器过滤器过滤器。请注意:将有条件的介质保持在 4°c, 以便短期储存, 或在-20°c 下存放超过1周。在稀释电镀之前准...