Zhang, N.; Sa, Y.; Guo, Y.; Wang, L.; Wang, P.; Feng, Y. Discriminating ramos and jurkat cells with image textures from diffraction imaging flow cytometry based on a support vector machine. Curr. Bioinform. 2018, 13, 50-56. [CrossRef]...
Jurkat cells treated with DPCPX underwent apoptosis as demonstrated by flow cytometry, by DNA fragmentation and by accumulation of histones, H2A, H2B, H3 and H4, in the nucleoplasm of cells. Cell cycle and cell sorting analyses indicated an arrest of cells in G(2)/M followed by the ...
MethodsAfter treated with different concentrations of Solanine, the proliferation of Jurkat cells was detected by CCK-8 assay, and the effect of Solanine on apoptosis of Jurkat cells were detected by flow cytometry.The expression of Bcl-2 and Bax in Jurkat cells were detected by Western blot,...
possible molecular mechanisms of curcumin-induced apoptosis. Methods Jurkat cells were treated with different concentrations of curcumin, and the cell proliferation and cell cycle changes were detected by MTT assay and flow cytometry, respectively. Western blotting and gelatin zymography were employed to e...
cells. The proliferation of the cells lines was assessed using CCK-8 assay, and the cell cycle distribution was determined by flow cytometry. The mRNA and protein expressions of cyclin-dependent kinase inhibitor 2 (CDKN2A) and cyclin-dependent ...
Human CD16A expression by Jurkat-Lucia™ NFAT-CD16 cells has been verified by flow-cytometry. These cells have been functionally tested with various target cells and specific monoclonal antibody (mAb) isotype combinations. Antibodies displaying lower EC50 have higher ADCC potency (Figures 1 & 2)...
induced apoptosis of Jurkat cells. 【Key words】 Jurkat; Taxol; Apoptosis; bcl-2 gene family 紫杉醇(paclitaxel,商品名Taxol)是从紫杉树皮中提取的一种新的抗微管药物[1],1993年美国FDA批准该药用于治疗对常规化疗无效的卵巢癌和乳腺癌,其作用是与微管结合使微管稳定性增强,抑制微管解聚,使细胞阻滞于分裂期...
Abstract; In order to explore the value ofγ-interferon (IFN-γ); in therapy of T lymphoid leukemia; and role of; cytometric bead array (CBA) method in detection of Th1/Th2 cytokines expression,the; Jurkat cells were cultured; for 48 hours; in; different; concentration; of; IFN-γ,the...
中文摘要: 目的 探讨徐长卿中天然产物XCQ-9抑制人急性T淋巴细胞白血病Jurkat细胞增殖和凋亡的作用及可能机制。方法以Jurkat细胞作为白血病细胞模型,采用MTT法测定0(空白对照)、2.5、5、10、20、40μmol/LXCQ-9作用24、48、72h后对Jurkat细胞增殖的抑制作用。用0(空白对照)、2.5、5、10μmol/LXCQ-9作用于Jurkat细...
Jurkat cells were tested for their susceptibility to apoptosis induced by TRAIL with TRAIL apoptosis kit so as to study the correlation between DR5 expression level and TRAIL sensitivity. The level of DR5 expression on Jurkat cell line was examined by flow cytometry. The rates of TRAILinduced ...