保存条件:液氮保存纯度规格:1x10(6)viable cells/ml 产品类别:实验化学品 "Jurkat Cell:人急性T淋巴细胞白血病细胞系 细胞形态:淋巴母细胞样 细胞生长:悬浮 细胞背景资料:该细胞源自一位14岁患有T淋巴细胞白血病男性的外周血 IGROV 1细胞系相关产品:HDF-a细胞、NCI-Hut 125细胞、OLN-93细胞 ...
Apoptosis in human Jurkat T cells after culture with live Taenia crassiceps cysticerci in vitro. Parasitology 2000;120(Pt 6):649-55.K.M. O'Connell, M.T. Rogan, Apoptosis in human Jurkat T cells after culture with live Taenia crassiceps cysticerci in vitro, Parasitology 120 (2000) 649...
Cultured cells ∙ Appropriate cell culture medium ∙ Purified plasmid DNA ∙ Serum-free medium (e.g. Opti-MEM ® I Reduced-Serum Medium) ∙ Sterile tube for transfection complex preparation ∙ Micropipets ∙ Reporter assay as required ∙ Optional: Selection antibiotic (e.g., ...
Spinning down the entire volume of cells greatly reduces the amount of debris in the culture. - At day 5 or 6, it is okay to add the selection. Continue to passage the cells (spinning the cells down) every 2-3 days for a total of 1.5 weeks, each time resuspending back into only ...
Synonym(s):Nuclear factor of activated T cells; T cell Freeze Medium:90% FBS+10% DMSO Culture Medium:RPMI-1640+10%FBS+800 μg/ml hygromycin Mycoplasma Testing:Negative Storage:Liquid nitrogen Application(s):Functional(Report Gene) Assay ...
索取Protocol I. Background 淋巴细胞活化基因 3 (LAG-3),又名 CD223,是一种 I 型跨膜蛋白,属于免疫球蛋白超家族 (IgSF)。LAG-3 蛋白与 CD4 有同源结构,两者的胞外区均具有四个 Ig 样结构域,但 LAG-3 在膜远端 Ig 样结构域内还含有一种额外的环状结构,使其与MHC II的亲和力较CD4高100倍。LAG-3 ...
别名:JURKAT, CLONE E6-1 Cells | JURKAT, CLONE E6-1 Human T-lymphocytic Leukemia Cell Culture Medium CAS番号: 分子式: 分子量:0 EINECS: Mol File:Mol File Browse by Nationality JURKAT, CLONE E6-1 Cells | JURKAT, CLONE E6-1 Human T-lymphocytic Leukemia Cell Culture Medium SuppliersGlobal sup...
Transfected cells were harvested 24 h later and prepared for luciferase assay. Firefly and pRL-TK-derived Renilla luciferase (RL) activities were measured according to the manufacturer's protocol with a Dual Luciferase Assay kit (Promega, Madison, WI, USA). The promoter activity was recorded as ...
Our objective was to determine the impact of mycophenolic acid (MPA) in comparison with rapamycin (Rapa), an inhibitor of mTORC1, on the metabolism of Jurkat T cells. We identified a drug-specific transcriptome signature consisting of the key enzymes and transporters involved in glycolysis, ...
Lipofectamine® LTX 试剂采用专有的无动物源性配方,用于将 DNA 转染到真核细胞中,且具有低细胞毒性。本参考文件提供了使用 Lipofectamine® LTX 试剂(货号 15338-100)将质粒 DNA 转染到人 Jurkat 人 T 细胞白血病细胞(ATCC 货号 TIB-152)中的推荐程...