In this example, Jurkat cells (1×106per sample) were rapidly pelleted bycentrifugationand alkylated as described for suspension cells above. MEFs were cultured in a 24-well plate and alkylated as described for adherent cells (A) above. In our experience with various cell types,Prxsare predomi...
migration of adherent cellsCell-IQ phase-contrast microscopycomputer morphometryT-lymphoblast-like human leukemia cells of the Jurkat line (Jurkat T cells) form polyploid forms with a high DNA content in suspension culture. Due to pronounced genetic instability, this contributes...
simulating devices. In our knowledge, it is hard to calculate or to generate a theoretical model to define possible disturbing mechanical stimuli during the functioning of microgravity simulators. This is due to the so many differences in cell models (adherent cells, cell suspension, tissue-like ...