We show here that PTEN potently inhibited the growth and reduced the size of Jurkat cells. The growth-suppressive effect of PTEN was associated with its ability to induce apoptotic cell death with little or no effect on cell cycle. PTEN also rendered Jurkat cells more susceptible to apoptosis ...
To study T-cell engagement in a ligand-dependent manner, we generated a human CD4-CD8αβ+ 1G4 TCR-expressing Jurkat T cell line42, transduced with additional LFA-1 to match the surface phenotype of primary CD8+ effector T cells (Supplementary Fig. 1f), and a genetically encoded calcium ...
Consistent with theory, Jurkat T cell growth rates increase with size for small cells, but decrease with size for large cells. In summary, our models show that regulation of both growth and cell-division timing is necessary for size control in animal cells, and this joint mechanism leads to ...
We found that the dynamics of the size distribution of Jurkat T-cells is reproduced by (i) a sizer-like division strategy, with (ii) division times following an Erlang distribution and (iii) fluctuations up to ten percent of the inherited fraction of size at division. Overall, our apparatus...
In Jurkat cells, the cell size and proliferation effects of statins were completely rescued by mevalonate, indicating that the effects are specific for the mevalonate pathway (Figure S4D). Cholesterol supplementation did not rescue cell size, as was expected because Drosophila cells are incapable of...
After the incubation, 50 mL of Jurkat cells washed by OptiMEM twice were added to the RNP solutions (final concentration of 13106 cells/mL) and shaken for 10 sec on a microshaker (M-BR- 024, TAITEC) gently. After 6 hours at 37uC, lactose (final concentration of 0.1 M) was added ...
Despite the wide size distribution across healthy cells, it has long been observed that the distribution of sizes within a single cell type is typically narrow [[3]]. Such a uniform distribution is a strong indicator that retaining a particular cell size is important for normal cellular function...
Minimal Impact on Live Cells The cytotoxicity of the materials used in Qtracker® Cell Labeling kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling kits appears to exert minimal impact on cellular...
Minimal Impact on Live Cells The cytotoxicity of the materials used in Qtracker® Cell Labeling kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling kits appears to exert minimal impact on cellular...
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays. 相关内容 3D Cell Culture Workflow Tools Explore our portfolio of 3D cell cult...