We show here that PTEN potently inhibited the growth and reduced the size of Jurkat cells. The growth-suppressive effect of PTEN was associated with its ability to induce apoptotic cell death with little or no effect on cell cycle. PTEN also rendered Jurkat cells more susceptible to apoptosis ...
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We found that the dynamics of the size distribution of Jurkat T-cells is reproduced by (i) a sizer-like division strategy, with (ii) division times following an Erlang distribution and (iii) fluctuations up to ten percent of the inherited fraction of size at division. Overall, our apparatus...
Consistent with theory, Jurkat T cell growth rates increase with size for small cells, but decrease with size for large cells. In summary, our models show that regulation of both growth and cell-division timing is necessary for size control in animal cells, and this joint mechanism leads to ...
Indeed, it has been shown that the amount of mitochondria in budding yeast19, HeLa20, mouse liver21, Jurkat and Drosophila Kc167 cells22 increases roughly in proportion to cell volume. In addition, the number of nucleoids in budding yeast correlates with mitochondrial network volume23, and ...
实验方案 T-Cell Migration Assays Using Millicell® Cell Culture Inserts Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.技术...
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays. 相关内容 3D Cell Culture Workflow Tools Explore our portfolio of 3D cell cult...
Minimal Impact on Live Cells The cytotoxicity of the materials used in Qtracker® Cell Labeling kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling kits appears to exert minimal impact on cellular...
Here, we assess the integration context dependence of expression heterogeneity from diverse single integrations of a HIV-promoter/GFP-reporter cassette in Jurkat T-cells. Systematically fitting a stochastic model of gene expression to our data reveals an underlying transcriptional dynamic, by which ...
After the incubation, 50 mL of Jurkat cells washed by OptiMEM twice were added to the RNP solutions (final concentration of 13106 cells/mL) and shaken for 10 sec on a microshaker (M-BR- 024, TAITEC) gently. After 6 hours at 37uC, lactose (final concentration of 0.1 M) was added ...