Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell density to exceed 3 X 10(6) cells/ml. ...
Here we present a method to characterize a dynamic secretome and demonstrate its utility by performing the deepest quantitative analysis to date of proteins secreted by lymphoid Jurkat T-cells upon activation. Cell-free supernatant proteins were analyzed by using an optimized protocol for differential ...
(B) The average number of GFP-LC3 puncta per cell. Each data point represents a total of 30 cells from five independent experiments for each protocol, means ± SD. ∗P < 0.01 compared with the control group. (C) Western blot analysis of p62 and LC3I/II in cells treated as described...
of live cells per well was determined by using the Cell Titer 96® Aqueous One Solution Cell Proliferation Assay with 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sul-fophenyl)-2H-tetrazolium (MTS) (Promega, Madison, WI, USA) according to the manufacturer’s protocol. ...
In conclusion, we created a novel iNKT reporter platform which, compared to traditional iNKT cell assays, is characterized by a shorter turnaround time and lower costs. It thus facilitates the identification of antigenic structures that drive the activation of iNKT cells in health and disease....
Thus, the GEF function of Vav1 is not required for T cell activation. This raises the question of the genuine cellular function of the Vav1 GEF. Our data demonstrated the requirement of the GEF activity of Vav1, but not Vav2 or Vav3, in inducing Bcl-2 transcription. Vav1 dose-...
询价 索取Protocol 产品描述 产品数据库I. Background Antibody-dependent cell-mediated cytotoxicity (ADCC) is an immune defense mechanism involving an effector cell lysing a target cell on which antibodies have bound to specific antigens on the target cell membrane. The typical ADCC involves ...
incombinationwiththemonitoringofcellviability,avaluabletooltodistinguishbetweenspecificimmuno-modulatoryandcytotoxiceffectsinvitro.KEYWORDS:RT-realtime-PCR;cytokines;JurkatTcells;NF- B;capsaicin;hypericin.INTRODUCTIONThesearchfornaturalproductswithanti-inflammatory,immunomodulatoryorantineoplasticpotentialhasalwaysbeenan...
Previous studies have documented the spontaneous aggregation of Fas receptor in lymphocytes upon treatment with UV light, leading to caspase activation (Rehemtulla et al., 1997). To examine if HCV core might similarly induce Fas multimerization, we employed an immunoprecipitation protocol using ...
Protocol 1. 基因组工程的目标策略与定位向量 (tv) 设计 请注意:基因组工程的第一步是选择和生成必要的工具, 以实现 crispr-cas9 介导的目标。在这一步之前, 应选择基因组整合位点, 选择用于定位的细胞类型, 以及设计一个 hiv 衍生的整合记者。该协议描述了针对一个 hiv-ltr _ tdTomato _ bgh-pa 最小...