以细胞群的平均红、绿荧光强度,计算红/绿荧光强度的比值(Ratio),根据重复实验数据,对Ratio进行统计,反映膜电位变化。 流式分析时,相对于正常对照,凋亡阳性处理的细胞群会往右下方发生移动,即红色荧光减弱,绿色荧光增强,这表明阳性处理导致线粒体膜电位下降。
Monitor the fluorescence change atEx/Em = 490/525 nm and 490/590 nm for ratio analysis. 3. Run JC-1 assay with a fluorescence microscope or a flow cytometer: Treat cells with test compounds for a desired period of time (For example, Jurkat cells can be treated with camptothecin for 4-...
However, in cells with low ΔΨM, JC-1 remains in the monomeric form, which exhibits green fluorescence. The higher the ratio of red to green fluorescence, the higher the polarization of the mitochondrial membrane.2Catalog Number KA
(λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and ...
The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green, while in healthy ...
按邮件里所述方法分别计算红、绿色荧光直方图(histogram)的数学均值(AM),以表示细胞群的平均荧光强度。计算Ratio值(Ration=AM红/AM绿),反映线粒体膜电位的平均水平。 20003589.snap.jpg hulu呼噜(2012-3-20 12:49:24) ——— 好像弄反了吧? FL2采集的是J-aggregates(富集于线粒体)的红色荧光,FL1采集的是...
Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨm induced by uncouplers of mitochondrial ...
JC-1 remains in a monomeric form, which exhibits green fluorescence. While in healthy cells with high MMP concentrations, JC-1 forms complexes known as J-aggregates with intense red fluorescence. The higher the ratio of red to green fluorescence, the higher the polarization of the mitochondrial...
JC-9 are cationic dyes (Figure 1) that exhibit potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (~525 nm) to red (~590 nm). Consequently, mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. ...
Changes in ΔψM reflected by different forms of JC-1 as either green or red fluorescence can be determined as a ratio of green:red using fluorescence microscopy or a fluorescence plate reader with appropriate filter sets. INTRODUCTION 5 PRE-ASSAY PREPARATION NOTE: JC-1 is light sensitive. Do...