The maximum excitation wavelength of JC-1 monomer is 514nm and the maximum emission wavelength is 529nm; the maximum excitation wavelength of JC-1 polymer is 585nm and the maximum emission wavelength is 590nm. For actual observation, use the conventional settings for observing red fluorescence an...
high, JC-1 aggregates in the matrix of the mitochondria to form a polymer, which can produce red fluorescence; when the mitochondrial membrane potential is low, JC-1 cannot accumulate in the matrix of the mitochondria, at this time JC- 1 is a monomer, which can produce green fluorescence....
Figure 2. NIH 3T3 fibroblasts stained with JC-1 showing the progressive loss of red J-aggregate fluorescence and cytoplasmic diffusion of green monomer fluorescence following exposure to hydrogen peroxide. Images show the same field of cells v...
The maximum excitation wavelength of JC-10 monomer is 515nm and the maximum emission wavelength is 529nm; the maximum excitation wavelength of JC-10 polymer is 585nm and the maximum emission wavelength is 590nm. For actual observation, use the conventional settings for observing red fluorescence ...