Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨm induced by uncouplers of mitochondrial ...
concentrations (aqueous solutions above 0.1 µM) or higher potentials, JC-1 dye forms red fluorescent "J-aggregates" where it has accumulated within the mitochondria (Figure 2). Consequently, mitochondrial depolarization is indicated by a ...
The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green, while in healthy ...
Consequently, mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The ratio of green to red fluorescence depends only on the mitochondrial membrane potential and not on other factors...
(λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) J-aggregates, which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not...
The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization. The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the ...
However, in cells with low ΔΨM, JC-1 remains in the monomeric form, which exhibits green fluorescence. The higher the ratio of red to green fluorescence, the higher the polarization of the mitochondrial membrane.2Catalog Number KA
The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density. 产品介绍 JC-1 是一种荧光亲脂性羰花青染料,可以用来衡量线粒体的膜电位。 别名 CBIC2;JC1;JC 1;5,5',6,6'-四氯-1,1',3,3'-四乙基...
The ratio of red-to-green JC-1 fluorescence is dependent only on the membrane potential and not on other factors that may influence single-component fluorescence signals, such as mitochondrial size, shape and density. Optical filters designed for fluorescein and tetramethylrhodamine (Table 23.12) ...
In healthy cells with high ΔψM, JC-1 forms complexes known as J-aggregates with intense red fluorescence. However, in cells with low ΔψM, JC-1 remains in the monomeric form, which exhibits green fluorescence. The higher the ratio of red to green fluorescence, the higher the ...