Simple, rapid methods allowing DNA to be released from bacterial cells, such as that described in Chapter 7, are sufficient for most PCR applications. Bacterial DNA can be prepared using extraction kits marketed by several manufacturers. Most of the kits use resins or membranes without organic ...
SUBSTANCE: DNA from microorganisms is isolated by disrupting bacterial cells with lysing solution containing 80% of phenol, 18.8% of H<Sub>2</Sub>O and 0.5% of NaOH (pH 9.3-9.5). Method provides preparing preparation of bacterial DNA with high purity and nativity degree. Invention can be ...
1. A method for isolating plasmids from suspended bacterial or yeast cells using a filter matrix, wherein at least one lysing substance is added to the suspension, said substance comprising, in predominant proportion, pre-damaged though not fully lysed cells, at least one conformation-altering sub...
coli DNA using a Retro-Cascorder Article 14 April 2023 Data availability The data that support the findings of this study are available from the corresponding authors upon request. Code availability ImageJ macros for image segmentation and MATAB scripts for cell tracking and signal processing ...
A rapid and simple procedure is described for obtaining CsCl-purified DNA from multiple small samples of cells or tissue. The DNA is recovered in a high-molecular-weight form (greater than or equal to 50 kb) that is readily cleaved with restriction enzymes. Sufficient quantities of DNA (10-...
A rapid and simple procedure is described for obtaining CsCl-purified DNA from multiple small samples of cells or tissue. The DNA is recovered in a high-molecular-weight form (≥50 kb) that is readily cleaved with restriction enzymes. Sufficient quantities of DNA (10–50 μg) are recovered ...
5′-CATCCTGTTAAGCTTACTGTCGCCCTTCGCCAGC-3′. To amplify linearised vector pNY326 DNA sequence, the following primers were used: forward, 5′-AAGCTTAACAGGATGCGGGG-3′; reverse, 5′-AGCGAAAGCCATGGGAGCAA-3′. The underlined sequences indicate overlap with both ends of insert fragment seque...
Nucleic acids found in cells can be deoxyribonucleic acid or ribonucleic acid and can be genomic DNA, extrachromosomal DNA (e.g. plasmids and episomes), mitochondrial DNA, messenger RNA and transfer RNA. Nucleic acids can also be foreign to the host and contaminate a cell as an infectious agen...
Processes for isolating, amplifying, and characterizing DNA from biological materials are provided. DNA is isolated by contacting a biological material on a solid support which is p
Levan from sucrose -Lecithinase -Urease -Hydrolysis ofStarch +Gelatine -Casein -DNA +Tween 80 +Aesculin +Tyrosine degradation+Utilisation ofsubstratesAcetate +Adipate -Caprate -Citrate -Glycolate -Lactate -Laevulinate -Malate +Malonate -Phenylacetate -Suberate -L-arabinose +Fructose +Glucose +...