Heterogeneity in single-cell RNA sequencing (scRNA-seq) datasets is frequently characterized by identifying cell clusters in gene expression space, wherein each cluster represents a distinct cell type or cell state. In particular, numerous studies have used unsupervised clustering to discover novel cell...
Bulk RNA sequencing provides the opportunity to understand biology at the whole transcriptome level without the prohibitive cost of single cell profiling. Advances in spatial transcriptomics enable to dissect tissue organization and function by genome-wide gene expressions. However, the readout of both t...
Right now since I don't add indels to the data, this string will either be a match or one of these chimeric jumps. About An illumina paired-end and mate-pair short read simulator. This project attempts to model as many of the quirks that exist in Illumina data as possible. Some of...
If you have paired-end Illumina sequencing data, processed by CASAVA v1.8+ the sequence identifiers for the pairs have the same id after truncating by space. To keep track of the individual reads throughout the HUMAnN 2.0 workflow, the software will initially remove the spaces from the identifi...
the total number of sequences in Illumina amplicon sequencing datasets, respectively. In square brackets, the percentage of 16S rRNA gene identity is reported for the plumeSulfurimonascluster.b, The two plots show the contribution of the hydrothermal plumeSulfurimonasecotypes (see Extended Data Fig.3...
We report that the hallmarks of tumor recognition in ovarian cancer-infiltrating T cells are primarily restricted to tissue-resident memory (TRM) cells. Single-cell RNA/TCR/ATAC sequencing of 83,454 CD3+CD8+CD103+CD69+ TRM cells and immunohistochemistry of 122 high-grade serous ovarian cancers ...
at the University of North Carolina using the Illumina HiSeq RNA Sequencing platform) for ARID1A in ccRCC and normal kidney and overall survival data were obtained from The Cancer Genome Atlas (TCGA), available through the cBio Cancer Genomics Portal (http://www.cbioportal.org/public-portal). ...
Centre at the University of North Carolina using the Illumina HiSeq RNA Sequencing platform) for LDHA in ccRCC and normal kidney and overall survival data were obtained from The Cancer Genome Atlas (TCGA), available through the cBio Cancer Genomics Portal (http://www.cbioportal.org/public-portal...
Preclinical data have confirmed that human pluripotent stem cell-derived cardiomyocytes (PSC-CMs) can remuscularize the injured or diseased heart, with several clinical trials now in planning or recruitment stages. However, because ventricular arrhythmia
In this scenario, many studies have recently revealed the importance of a new member of the noncoding RNA clade, termed long-noncoding RNAs (lncRNAs), in the regulation of gene activity10,11. In particular, the FANTOM (Functional Annotation of the Mammalian Genome) consortium pioneered the disc...