2001. The first product of phospholipid N-methylation, phosphatidylmonomethyletha- nolamine, is a lipid mediator for progesterone action at the amphibian ... AB Kostellow,GY Ma,GA Morrill - 《Steroids》 被引量: 13发表: 2001年 The first product of phospholipid N-methylation, phosphatidylmonomethy...
we repeated the experiment in THP-1 cells in which we reduced the expression of RNF213 by siRNA. As expected, knockdown of RNF213 markedly reduced association of ISGylated proteins with LDs and this occurred without any apparent loss of lipid droplet stability as indicated by unchanged ...
Apolipoprotein E4 (APOE4) is the strongest known genetic risk factor for late-onset Alzheimer’s disease (AD). Conditions of stress or injury induce APOE expression within neurons, but the role of neuronal APOE4 in AD pathogenesis is still unclear. Here
Renal cortex was dissected from the outermost 3 mm of the kidney perimeter on ice-cold glass plates and homogenized in an ice bath in 10 volumes of homogenization buffer [250 mmol/L mannitol, 70 mmol/L sucrose, 1 mmol/L ethylenediaminetetraacetic acid (EDTA), adjusted with Tris to pH = ...
macromeris was susceptible to callus induction by using a medium rich in sucrose (20 g L−1) and BAP combined with 2,4-D. Nevertheless, information regarding the induction percentages, and the characteristics of the formed tissue and its kinetic behavior was not given. For other Coryphantha...
Downregulated expression of starch biosynthesis genes and upregulated expression of sucrose cleavage genes might be a result of reduced photosynthetic activity and account for the decreased starch and sucrose levels seen in phd1 leaves. PHD1 overexpression increased photosynthetic efficiency, biomass, and ...
The samples were allowed to solidify on ice and were infiltrated with 2.3M sucrose at 4C overnight, mounted onto sample pins and frozen in liquid nitrogen. Subsequently, the samples were cryo- sectioned (60 nM thickness) using a FC6/UC...
We identify an endogenous role for PPARα in retinal neuronal survival and lipid metabolism, and furthermore underscore the importance of fatty acid oxidation in photoreceptor survival. We also suggest PPARα as a putative therapeutic target for age-related macular degeneration, which may be due in...
Surface-sterilized seeds were sown on agar- solidified Murashige and Skoog medium53 containing 1% (w/v) sucrose at 22 °C under 16 h light/8 h dark cycles. Photon flux density was 50 mmol m–2 sec–1. The seedlings were kept on agar for 14 days before transfer to plates with ...
Cells were disrupted by nitrogen cavitation and subjected to sucrose density gradient ultracentrifugation as described (2). The lipid droplet layer floating on the surface was used in subsequent studies. Absence of contamination by other organelles was confirmed by Western blotting of marker molecules. ...