The proton-sucrose symporter mediates the key transport step in the resource distribution system that allows many plants to function as multicellular organisms. In the results reported here, we identify sucrose as a signaling molecule in a previously undescribed signal-transduction pathway that regulates...
The OGTT consists exclusively of glucose, whereas the High Carb meal consists of sucrose, which is composed of one molecule of glucose, and one molecule of fructose. Unlike glucose in the OGTT, the fructose represented in the High Carb meal results in a more modest effect on blood glucose ...
Apolipoprotein E4 (APOE4) is the strongest known genetic risk factor for late-onset Alzheimer’s disease (AD). Conditions of stress or injury induce APOE expression within neurons, but the role of neuronal APOE4 in AD pathogenesis is still unclear. Here
the corresponding human residues are shown in orange and mole residues are shown in purple. Catalytic residues are shown in cyan and glycosylated residues in green. A molecule of HA tetramer (yellow) from bee venom HYAL (PDB code
The samples were allowed to solidify on ice and were infiltrated with 2.3M sucrose at 4C overnight, mounted onto sample pins and frozen in liquid nitrogen. Subsequently, the samples were cryo- sectioned (60 nM thickness) using a FC6/UC...
Proteolytic cell surface release (‘shedding’) of the prion protein (PrP), a broadly expressed GPI-anchored glycoprotein, by the metalloprotease ADAM10 impacts on neurodegenerative and other diseases in animal and in vitro models. Recent studies employing the latter also suggest shed PrP (sPrP) to...
Groups of 20–25 one- to two-day-oldflies were starved for 4 h and then kept in vials with filter paper soaked with 5 mm PQ (plus 200 μm to 1 mm M40403 when required) in 5% sucrose. Surviving to the chemical treatment was determined every day for a 4 day-period. Experiments we...
The cells were collected by centrifugation at 3,000 g and re-suspended in 1/2 MS0 medium containing 2.21 g MS powder and 30.00 g sucrose per liter, with acetosyringone and 2-(N-Morpholino) ethane sulfonic acid(MES) to a final concentration of 40 μM and 10 mM. The re-suspended ...
buffer containing sugars and other compounds was therefore tested. Glucose, fructose, sucrose, and the small sugar alcohol glycerol eluted FilP with comparable efficiency (Fig.4f). The heterocyclic molecule imidazole was used as a control for cyclic compounds such as sugars and did not elute FilP....
For Oil red O staining, liver tissues were fixed in 4% paraformaldehyde in phosphate buffered saline, then changed to 30% sucrose overnight, frozen sectioned and stained with Oil Red O. Isolation of mitochondria Mitochondria were isolated from the mouse livers by differential centrifugation. Mouse...