Herein, we show increased levels of double-stranded RNA (dsRNA) in infected bone in a Staphylococcus-induced chicken BCO model and in human osteomyelitis samples. Administration of synthetic [poly(I:C)] or genetic (Alu) dsRNA induces human osteoblast cell death. Similarly, infection with ...
Application Brief: Characterization of a Synthetic Double Stranded siRNA using High-Resolution Mass Spectrometry Application Note: Simultaneous Analysis of Mycotoxins in Corn-Based Animal Feed by Quadrupole-Orbitrap LC-MS/MS Case Study: EUROLAB Adopts Orbitrap High-resolution Accurate-Mass (HRAM) Solution...
L. (2012). Gene silencing mediated by siRNA-binding fusion proteins is attenuated by double- stranded RNA-binding domain structure. Mol. Ther. Nucleic Acids. 1:e53. doi: 10.1038/mtna.2012.43Geoghegan JC, Gilmore BL, Davidson BL: Gene Silencing Mediated by siRNA- binding Fusion Proteins Is ...
siRNAs也许被设计作为对称分子与感觉和antisense子线有同一长度。 double-stranded区域也许变化在19和21核苷酸之间。 二核苷酸突出物长在任一条子线的3 ′末端是有利的。 除非数据关于siRNA的最佳的G+C内容引起,它是慎密避免极端价值。 所以, double-stranded区域的G+C内容应该在40%到55%之间。
What is the difference between (+) and (-) stranded RNA viruses? How do DNA and RNA differ? a. DNA is double-stranded while RNA is single-stranded. b. They have different sugars. c. Thymine is present in DNA but not in RNA. d. All statements are differences between DNA and RNA. ...
Oncogene (2017) 36, 1840–1848; doi:10.1038/onc.2016.347; published online 10 October 2016 INTRODUCTION One of the most life-threatening events that can occur to cells that are preparing to divide is a double-stranded break in their DNA. In order to deal with such an event, cells activate...
Double-stranded siRNAs were purchased from Eurofins Genomics (Tokyo, Japan). Their sequences were: siBaf53a #1: sense 5′-GGA CUG CCC UAA GGU UGA UUU-3′ and antisense 5′-AUC AAC CUU AGG GCA GUC CUC-3′; siBaf53a #2: sense 5′-GGC CAU UUU GGA UCA UAC AUA-3′ and antisense ...
siRNAs are highly specific and usually synthesized to reduce the translation of specific messenger RNAs (mRNAs). This is done to reduce the synthesis of particular proteins. They form from double-stranded RNA transcribed and then cut to size in the nucleus before releasing into the cytoplasm. ...
Double-stranded RNA (dsRNA) induces sequence-specific posttranscriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 21- and 22-nt RNA fragments are the sequence-specific mediators of RNAi. The short int...
Here, we show that CD14 enhances double-stranded RNA (dsRNA)-mediated Toll-like receptor 3 (TLR3) activation. Bone marrow-derived macrophages (BMDMs) from CD14−/− mice exhibited impaired responses to polyinosine-polycytidylic acid (pIpC) and reduced production of inflammatory cytokines. CD14...