This repositioning enables directional binding of RNA duplexes, thereby biasing their orientation for guide strand selection according to the thermodynamic properties of the helix. Our findings indicate that Dicer is itself capable of sensing siRNA thermodynamic asymmetry regardless of the dsRBP to which...
Ago2 uses one strand of the small interfering (si) RNA duplex as a guide to find messenger RNAs containing complementary sequences and cleaves the phosphodiester backbone at a specific site measured from the guide strand's 5′ end. Here, we show that both strands of siRNA get loaded onto ...
Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation Cell, 123 (2005), pp. 621-629 View in ScopusGoogle Scholar Rissland et al., 2011 O.S. Rissland, S.J. Hong, D.P. Bartel MicroRNA destabilization enables dynamic regulation of the miR-16 family in response to cell...
It is seen that there is perfect base pairing in siRNA, however, miRNA duplexes exhibit flawed pairing between the sense (passenger) and antisense (guide) strand. The guide strand gets incorporated into the RISC guiding it to the target mRNA and inducing gene silencing effect, however, the ...
Transcription-based sample preparation methods.aT7 in vitro transcription scheme; the double-stranded (dsDNA) T7 promoter sequence and 5′-methoxy modification of the DNA template strand are highlighted.bPosition-selective labeling of RNA in the solid and solution phase (left). Fundamental steps in ...
A trans-type substrate structure, composed of the gRNA and target RNA, would not involve a phosphodiester bond between the strand con- taining a target-site and the ARR. Hence, the relative distance and direction of the ARR from the target-site should be flexible. We hypothesized that AD-...
Programmable nickases derived from nucleases induce single-strand breaks (that is, 'nicks'), the repair of which can lead to precise genome editing. Paired nickases can induce genome editing as efficiently as nucleases but with a much higher specificity. With programmable nucleases, artificial selec...
(e-sgRNA) and mRNA encoding Cas9, we show that a single intravenous injection into mice induces >80% editing ofPcsk9in the liver. Serum Pcsk9 is reduced to undetectable levels, and cholesterol levels are significantly lowered about 35% to 40% in animals. This strategy may enable non-viral...
However, the expression and function of miR-21-3p (passenger strand) are not well understood. We found that upregulation of miR-21-5p and miR-21-3p tune purine metabolism leading to increased drug tolerance. Metabolomics data demonstrated that purine metabolism was the top pathway in the DTP...
The heteroduplex maintains Watson–Crick base-pairing even in the 3′-region of the guide RNA between the N-terminal and PIWI domains, suggesting a recognition mode by RsAgo for stable interaction with the target strand. In addition, the MID/PIWI interface of RsAgo has a system that ...